FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396873
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA32130.8095788829172051No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16420.4137343684251637No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC12090.304631456410489No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11450.28850539089330846No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11040.2781746301713647No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10740.27061553696018625No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10590.26683599035459704No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10140.25549735053782946No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9400.23685158728358946No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9360.23584370818876568No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9260.2333240104517062No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9200.23181219180947055No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC8720.21971764267158514No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8640.21770188448193756No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8400.21165460991299487No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8210.20686718421258185No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7940.2000640003225213No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT6890.17360717408339696No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC6840.17234732521486723No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6820.17184338566745533No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6590.1660480808722186No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6370.16050474585068775No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA6300.15874095743474612No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6270.1579850481136283No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC6090.15344959218692125No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5780.14563852920203693No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5590.14085110350162394No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5360.13505579870638718No Hit
GTGCTGGTGGGAGTGTAGCAGTGAGGACGACCAGAAGTCACTCTCGTGGC5160.13001640323226826No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5080.1280006450426207No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4950.12472503798444337No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4910.1237171588896196No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4850.12220534024738393No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4770.12018958205773636No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4750.11968564251032446No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT4650.117165944773265No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4640.11691397499955906No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG4620.11641003545214716No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG4610.11615806567844122No Hit
CTTTAGGACATGGACATTGATCCCTATAAAGAATTTGGAGCTTCTGTGGA4610.11615806567844122No Hit
ATGTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA4600.11590609590473527No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTTCCTGTC4600.11590609590473527No Hit
TCCTCAGGCAATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG4530.11414230748879364No Hit
GTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT4510.11363836794138174No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC4470.11263048884655796No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA4410.1111186702043223No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC4380.11036276088320444No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA4310.10859897246726283No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTAG4310.10859897246726283No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA4310.10859897246726283No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGAGACTGGATCTTG4290.10809503291985094No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4230.10658321427761525No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGCGAGTCCAA4210.10607927473020337No Hit
GAGATACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAGCCACCA4200.10582730495649743No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4200.10582730495649743No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4140.10431548631426174No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGATCACCATATT4100.10330760721943795No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT4000.10078790948237851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)