FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614396
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145452.3673656729536No Hit
TCCACAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGA34830.5668982219936327No Hit
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGA26260.4274116367945104No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT17130.2788104089219331No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA16110.26220873833814023No Hit
AAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGTCAAT11730.19091921171361792No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10930.17789829360868234No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10790.17561963294031863No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10450.170085742745721No Hit
CAATAGGCCTGTTAACTGGAAGTTTTCTGAAACATTCTTTGATTTTCTGT10420.1695974583167859No Hit
CATTAGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCAT10370.16878365093522743No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9990.16259871483538305No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT9820.15983176973808422No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9460.15397235659086322No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGCTACTCTTTACATTTCAT9360.1523447418277463No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8990.14632256720421358No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8540.1389983007701873No Hit
GTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8500.1383472548649405No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTT8160.1328133646703429No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT7900.12858156628623885No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7840.12760499742836867No Hit
GGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTTCAGG7750.1261401441415634No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTTTCA7710.12548909823631663No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC7670.12483805233106987No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA7430.12093177689958919No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG7210.1173510244207319No Hit
CACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6960.11328198751293954No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6930.11279370308400445No Hit
CTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTT6790.11051504241564074No Hit
GTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6780.11035228093932903No Hit
TATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGGCATA6750.10986399651039394No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6740.10970123503408226No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA6640.1080736202709653No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6640.1080736202709653No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTTTCATTGAC6580.10709705141309514No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACATTATT6330.10302801450530277No Hit
CTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTAGTTT6270.1020514456474326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)