FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433951
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81621.8808575161711805No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA12010.27675935762332615No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8990.20716624688040816No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT8850.2039400761837166No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8630.19887037937462984No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7820.18020467748662866No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT7740.1783611513742335No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7680.1769785067899371No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7600.17513498067754193No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7580.17467409914944312No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7340.16914352081225761No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7130.16430426476722026No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACAACCGTG6480.14932561510400943No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6360.14656032593541665No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT6180.14241239218252752No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6130.14126018836228055No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5690.13112079474410704No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5670.13065991321600826No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5630.12973815015981066No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5560.12812506481146488No Hit
CCACCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5460.1258206571709709No Hit
ATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGA5270.12144228265403237No Hit
GTCAATGAGAAACGAAGACTGAAATTGATTATGCCTGCTAGGTTTTATCC5090.11729434890114321No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4820.11107244827180948No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4720.10876804063131551No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCGACCAATCGTCAGTC4610.10623319222677215No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTACTGTGGTTTCACATTTCTT4510.10392878458627818No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4510.10392878458627818No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA4440.1023156992379324No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4430.10208525847388299No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4350.10024173236148781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)