FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433951
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81491.8778617862385387No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10810.24910646593739846No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8550.19702685326223468No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8460.19495288638579011No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8220.18942230804860455No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8190.1887309857564564No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8020.1848134927676166No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT7710.1776698290820853No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7490.17260013227299856No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACAACCGTG7070.1629216201829239No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7040.1622302978907757No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT7000.1613085348345781No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT7000.1613085348345781No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT6720.15485619344119497No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6620.152551785800701No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6370.14679076669946609No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6340.14609944440731787No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT6000.1382644584296384No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5970.13757313613749017No Hit
CCACCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5310.12236404571022996No Hit
ATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTTGGA5130.1182161119573408No Hit
GTCAATGAGAAACGAAGACTGAAATTGATTATGCCTGCTAGGTTTTATCC5050.11637258584494563No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5010.11545082278874805No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4710.10853759986726612No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4620.10646363299082154No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4600.10600275146272274No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4560.10508098840652516No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGA4550.10485054764247577No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC4520.10415922535032757No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4490.10346790305817938No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTACTGTGGTTTCACATTTCTT4430.10208525847388299No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCGACCAATCGTCAGTC4420.1018548177098336No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4400.1013939361817348No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC4340.10001129159743843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)