FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419316
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGG24860.5928702935256466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17290.4123381888599529No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15930.3799044157628137No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT13570.3236222800942487No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11860.2828415800971105No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCC11160.2661477262971124No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA10980.2618550210342558No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT10490.2501693233742571No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT8810.2101040742542617No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8710.2077192379971191No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT8210.19579505671140618No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7940.1893559988171212No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7760.18506329355426457No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7390.176239399402837No Hit
GTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACC7080.16884640700569498No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTT7010.16717702162569517No Hit
CAATAGGCCTGTGAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6920.16503066899426685No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6240.1488137824456973No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6220.14833681519426878No Hit
ACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTCACAGG6060.14452107718284063No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5970.1423747245514123No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5790.13808201928855565No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT5670.13522021577998455No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5630.1342662812771275No Hit
GTTCCGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5530.13188144501998494No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5490.1309275105171279No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5380.12830419063427104No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5350.12758873975712828No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG5300.126396321628557No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5170.12329603449427162No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTT5170.12329603449427162No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5040.12019574735998627No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4960.11828787835427218No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGAAGTTATGGGTCATTGCCAC4890.11661849297427238No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGG4890.11661849297427238No Hit
ATATATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG4810.11471062396855831No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4790.1142336567171298No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4770.11375668946570128No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT4710.11232578771141573No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA4630.11041791870570167No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4610.10994095145427316No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA4520.10779459882284481No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4520.10779459882284481No Hit
TTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACCAGACATTATT4510.10755611519713056No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4510.10755611519713056No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4500.1073176315714163No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4450.106125213442845No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4420.10540976256570224No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4420.10540976256570224No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCATTGAC4370.10421734443713095No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4350.10374037718570243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)