FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419316
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGG22160.5284797145827967No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17320.41305363973709563No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15430.3679802344771008No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT14220.3391237157656755No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11080.2642398572913984No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCC10970.2616165374085415No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT10170.24253784735140085No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA9860.23514485495425885No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGGAT9210.21964341928283204No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8710.2077192379971191No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACT8440.20128018010283416No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8210.19579505671140618No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7810.18625571168283586No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7400.17647788302855127No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7150.1705157923856948No Hit
CAATAGGCCTGTGAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6740.1607379637314102No Hit
GTATTAAACCTTATTATCCAGAACACCTAGTTAATCATTACTTCCAAACC6550.1562067748428393No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT6160.14690591343998322No Hit
ACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTCACAGG6070.14475956080855487No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5980.14261320817712655No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5900.14070533917141248No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5660.13498173215427028No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTT5500.13116599414284216No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5460.1302120596399851No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5420.1292581251371281No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGAAGTTATGGGTCATTGCCAC5320.1268732888799855No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGGTGTTCTGG5270.1256808707514142No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA5240.12496541987427143No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5180.12353451811998588No Hit
GTTCCGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5160.12305755086855737No Hit
ATATATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAG4980.11876484560570072No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT4970.11852636197998646No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4830.11518759121998683No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT4830.11518759121998683No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4820.11494910759427257No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4790.1142336567171298No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4780.11399517309141555No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4760.11351820583998702No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4660.11113336958284445No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTT4660.11113336958284445No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4600.1097024678285589No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG4590.10946398420284464No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4520.10779459882284481No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCATTGAC4500.1073176315714163No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC4490.10707914794570204No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4460.10636369706855926No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4450.106125213442845No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4430.1056482461914165No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4360.10397886081141669No Hit
GTCATAGAGTATTTGGTGTCTTTCGGGGTGTGGATTCGCACTCCTCCAGC4330.10326340993427391No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT4260.1015940245542741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)