FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642557
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88001.3695283064381838No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA32990.5134174866976782No Hit
GGATAAGGGTATCAAACCTTATTATCCAGAACATCTAGTTAATCATTACT14140.2200582983299536No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13250.20620738704893107No Hit
CATTTAAACCCTAACAAAACAAAACGATGGGGTTACTCTTTACATTTCAT13140.20449547666588336No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT12900.20076039946650648No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCCAATACAAAGATC11720.18239626990290356No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11230.17477048728750913No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10560.16434339677258203No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10320.16060831957320518No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10110.15734012702375041No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCGAAAACATTCTTTGATTTTCTGT9660.1503368572749188No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9390.14613489542561983No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8960.1394428821100696No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8800.13695283064381836No Hit
AGTCTGTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8630.13430715096092644No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8420.13103895841147165No Hit
GGATAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATT8340.12979393267834605No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATCAAACCTTAT7970.12403568866264006No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7930.12341317579607723No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7740.12045623967990389No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7610.11843307286357475No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7470.11625427783060491No Hit
ATGTGGTATTGGGGGCCAAGTCTGTATCGCATCTTGAGTCCCTTTTTACC7380.1148536238808386No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7220.11236357241458735No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA6980.10862849521521048No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTATCGCATCTTG6970.10847286699856978No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG6940.10800598234864767No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6910.10753909769872558No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6820.10613844374895924No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6780.10551593088239641No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6710.10442653336591151No Hit
ATTTATGCCTACAGCCTCCCAATACAAAGATCATTAATCTAGTCTCCTCC6690.1041152769326301No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6660.10364839228270799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)