FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603110
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42290.701198786291058No Hit
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT20120.3336041518131021No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA15940.26429672862330256No Hit
ATATAAGACCTTGGGCAATATTTGGTGGGCGTTCACGGTGGTCTCCATGC14780.24506308965197063No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14230.2359436918638391No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATGGGAAAAAAT12530.20775646233688713No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT12420.20593258277926083No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12310.20410870322163452No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGAT11770.19515511266601449No Hit
TTCTAAAACAGTCTTTGATTTTCATTCTGATGTGTTCCTGTGGTAATGAT11460.19001508845815854No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACTCCCGTG11360.18835701613304373No Hit
TCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGA10950.18155891960007295No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT10850.17990084727495814No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA10580.1754240519971481No Hit
TCCAAAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA10120.16779691930161994No Hit
ACATTATCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTT9810.162656895093764No Hit
CTGTTAGAGGACCCACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC9680.1605014010711147No Hit
TAATAACTCTATCTACCTGGGTGGGTGCAAATTTGGAAGATCCAGCATCC9470.1570194491883736No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC9420.15619041302581618No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC9210.15270846114307507No Hit
ATACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCT9140.15154781051549468No Hit
TCCACAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA8920.14790005140024207No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8320.13795161744955314No Hit
CCTTACACTTGGGAACAAGAGCTACATCATGGGGCTTTCTTGGACGGTCC8130.13480128003183497No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGATA8120.1346354727993235No Hit
CTTTATACCTCTGTGACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7870.13049029198653644No Hit
GGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGAT7800.12932964135895608No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAATATTTGGTGGGCGTTC7630.12651091840626089No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7570.12551607501119197No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCCAACAAA7320.12137089419840494No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7160.11871797847822121No Hit
GTATAAAACCTTATTATCCAGATAATGTAGTTAATCATTACTTCCAAACC7110.1178889423156638No Hit
TTATTAACAAATGTGAACAATTTGTGGGTCCTCTAACAGTAAATGAAAAA6790.11258311087529638No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGG6770.1122514964102734No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT6750.11191988194525046No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6400.10611662880734857No Hit
TTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC6380.10578501434232561No Hit
CTACATTATCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATAT6250.10362952031967634No Hit
CTCCAAAAGACACCAAATACTGTACGACGGTTTCTCTTCCAAAAGTGAGA6190.10263467692460744No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTAAG6150.10197144799456152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)