FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603110
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101411.6814511448989404No Hit
CTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT17640.2924839581502545No Hit
TCCATAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA14170.2349488484687702No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGATGGGAAAAAAT14050.2329591616786324No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12390.20543516108172638No Hit
ATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGAT12330.2044403176866575No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACTCCCGTG12320.204274510454146No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11990.1988028717812671No Hit
CTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT11120.18437764255276815No Hit
TTCTAAAACAGTCTTTGATTTTCATTCTGATGTGTTCCTGTGGTAATGAT10650.17658470262472847No Hit
ACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATAT10410.17260532904445292No Hit
CTCTAACAGTAAATGAAAAACGAAGATTAAACTTAGTCATGCCTGCTAGA9900.16414916018636733No Hit
CTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC9810.162656895093764No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9290.1540349190031669No Hit
TCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGA9230.15304007560809801No Hit
ATATAAGACCTTGGGCAATATTTGGTGGGCGTTCACGGTGGTCTCCATGC9200.1525426539105636No Hit
TCCAAAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA9150.15171361774800618No Hit
CTGTTAGAGGACCCACAAATTGTTCACATTTGTTAATAATGTCCTGGTGC9120.1512161960504717No Hit
ACATTATCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTT9100.15088458158544876No Hit
ATACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCT8780.14557875014508131No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC8690.144086485052478No Hit
GGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGAGAT8420.13960968977466795No Hit
TAATAACTCTATCTACCTGGGTGGGTGCAAATTTGGAAGATCCAGCATCC8280.13728838851950723No Hit
TCCACAGGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACA8250.13679096682197275No Hit
CTTTATACCTCTGTGACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7540.12501865331365755No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7480.12402380991858866No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGG7410.12286315929100827No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCCAACAAA7300.12103927973338197No Hit
CCTTACACTTGGGAACAAGAGCTACATCATGGGGCTTTCTTGGACGGTCC7060.1170599061531064No Hit
TTATTAACAAATGTGAACAATTTGTGGGTCCTCTAACAGTAAATGAAAAA6900.11440699043292268No Hit
GCTTTACTCTTCTACTATACCTGTCTTTAATCCTAACTGGAAAACTCCAT6830.11324633980534231No Hit
TGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCTGGATA6760.11208568917776193No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6600.10943277345757821No Hit
TTCTTGGACGGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC6380.10578501434232561No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6110.10130821906451559No Hit
GGGTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACATTATCT6100.10114241183200412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)