FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116886
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7710.6596170627791181No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3730.3191143507349041No Hit
GCGTCCAGTTTCTCTTTTATATAAGATGCCCGCCTTCCACAGGGTATGTA3630.3105590062111801No Hit
ATCACGGTATGTTACCCGTTTGTCCTCTAATTCCAAGATCATCAACCAAC3120.2669267491401879No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3060.2617935424259535No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG2800.2395496466642712No Hit
GCTGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTATGAC2730.23356090549766442No Hit
GTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC2700.2309943021405472No Hit
GTCACTTATGTCAATACTAATATGGGCTTAAAGTTCAGGCAATTATTGTG2680.2292832332358024No Hit
TCTACTCTCCTCCCCCGACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA2670.22842769878343No Hit
CTTCTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT2560.21901681980733365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2430.20789487192649247No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA2370.2027616652122581No Hit
ACATCGACCCTTATAAAGAATTTGGCGCTTCTGTGGAGTTACTCTCTTTT2180.18650651061718257No Hit
GGGTCTTTCTTGTTGACAAAAATCCTCACAATACCACAGAGTCTAGACTC2120.18137330390294817No Hit
GTGCTGGTGGGAGTGTAGCAGTGAGGACGACCAGAGGTCACTCTCATCAC2070.1770956316410862No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT2040.174529028283969No Hit
CAATCACATAACCCATGAAATTTAGGGAATATCCCCATCTTTTTGTTTTG2030.17367349383159658No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGATGTGGGAAAAAAT2010.1719624249268518No Hit
CTGTCCAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTG1940.16597368376024502No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA1830.15656280478414866No Hit
GGGACGGGTCGTCCGCAGGATTCAGCGCTGACGGGACGTAGACAAAGGAC1810.15485173587940387No Hit
GCTCTGTATTATGAATGGAAATCAGAGGAAAAAGAGATTTATAAGAGTCT1750.14971852916516948No Hit
GGGTCTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTC1740.1488629947127971No Hit
GGTCAGTAATCTACTCTCCTCCCCCGACTCCTCCCAGTCTTTAAACAAAC1710.14629639135567987No Hit
CTCCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCG1690.1445853224509351No Hit
CCTTAGAATCTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCAA1660.1420187190938179No Hit
GTATTATGAATGGAAATCAGAGGAAAAAGAGATTTATAAGAGTCTTTTAA1660.1420187190938179No Hit
GTCCATGTACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA1640.1403076501890731No Hit
GGGAGGGAGCCCTCAGGCTCAAGGCATGCTAAAAACAATGCCAGCAGATC1560.13346337457009394No Hit
GTTCCTGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA1520.13004123676060433No Hit
CCTTTATACCGCTGTTACTAATTTTCTTTTGTCTTTGGGTATACATTTAA1510.12918570230823195No Hit
GGGGTGGAGCCCTCAGGCTCAAGGCATGCTAAAAACAATGCCAGCAGATC1500.12833016785585954No Hit
GCTCAGCTCTGTATTATGAATGGAAATCAGAGGAAAAAGAGATTTATAAG1490.12747463340348716No Hit
TTCTTGGACAGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC1490.12747463340348716No Hit
ATTTTACACAGCCCCTATTCAAGATGGAGTTGCTCTAGTTCAACTGCCTC1490.12747463340348716No Hit
CTCCCACTCCTACCTTGTTGGCTTCTGTCCAGTGGTCTTTGTTGGGATTG1480.12661909895111476No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT1460.12490803004636998No Hit
CTCCACCCCAAAAGGCCTCCGTGTGGGGGAGTGAACCCCGGCCCGAATGC1460.12490803004636998No Hit
TTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGT1460.12490803004636998No Hit
GCTTTACTCTTCTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCAT1420.12148589223688039No Hit
CTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT1420.12148589223688039No Hit
ATGTAATTGGAAGTTGGGGATCATTACCACAGGAACACATCAGAATGAAA1410.120630357784508No Hit
GTTCCTCGAGCAAGAGTCATGCAGGTTCGGCAGGTTCCCGTACTGTTGGT1410.120630357784508No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT1400.1197748233321356No Hit
GCCTTAGAATCTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCA1390.11891928887976318No Hit
GTTCCTCGAGCAAGAGTCGTGCAGGTTCGGCAGGGTCCCGTACTGGTGGT1380.11806375442739078No Hit
TCCCCAAGCGACCCCGGGACGGGTCGTCCGCAGGATTCAGCGCTGACGGG1380.11806375442739078No Hit
CTCAAGAAACCTCTATGTTTCCCTCATGTTGCTGTTCAAAACCTTCGGAC1380.11806375442739078No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC1370.11720821997501839No Hit
ACCCATCTCGGGGTCGCTTAGGGATCTATCGTCCCCTTCTCCGTCTGCCG1340.1146416166179012No Hit
ATCCAAATAATATAGTTAATCATTACTTCCAAACCAGACACTATTTACAT1340.1146416166179012No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGCGTCC1340.1146416166179012No Hit
CTCCAAAAGACACCAAATACTCTATGACGGTTTCTCTTCCAAAAGTGAGA1300.11121947880841161No Hit
GGTCCATGTACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG1280.10950840990366682No Hit
CTATTTACATACCCTGTGGAAGGCGGGCATCTTATATAAAAGAGAAACTG1260.10779734099892202No Hit
ATTCAAGGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCTT1200.10266413428468765No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAG1200.10266413428468765No Hit
CTCTTCTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC1200.10266413428468765No Hit
ATACTAATATGGGCTTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCT1190.10180859983231524No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC1180.10095306537994285No Hit
CATCGACCCTTATAAAGAATTTGGCGCTTCTGTGGAGTTACTCTCTTTTT1170.10009753092757046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)