FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116886
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5640.482521431138032No Hit
GCGTCCAGTTTCTCTTTTATATAAGATGCCCGCCTTCCACAGGGTATGTA3190.27291549030679463No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG2980.25494926680697433No Hit
ATCACGGTATGTTACCCGTTTGTCCTCTAATTCCAAGATCATCAACCAAC2970.2540937323546019No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA2900.24810499118799512No Hit
GGGTCTTTCTTGTTGACAAAAATCCTCACAATACCACAGAGTCTAGACTC2670.22842769878343No Hit
CTTCTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCT2640.2258610954263128No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2600.22243895761682322No Hit
ACCCATCTCGGGGTCGCTTAGGGATCTATCGTCCCCTTCTCCGTCTGCCG2570.21987235425970603No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2400.2053282685693753No Hit
ACATCGACCCTTATAAAGAATTTGGCGCTTCTGTGGAGTTACTCTCTTTT2310.1976284584980237No Hit
GCTGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTATGAC2310.1976284584980237No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT2300.19677292404565133No Hit
GTCACTTATGTCAATACTAATATGGGCTTAAAGTTCAGGCAATTATTGTG2220.18992864842667215No Hit
GTGCTGGTGGGAGTGTAGCAGTGAGGACGACCAGAGGTCACTCTCATCAC2190.18736204506955495No Hit
GTTTTATACCTTTCTCTAGGGGCAAATATTTCGTAGATGTGGGAAAAAAT2160.18479544171243775No Hit
GTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC2050.1753845627363414No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT2020.1728179593792242No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA1970.16854028711736221No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGAGGG1910.16340708040312782No Hit
GGGTCTTTCTTGTTGACAAAAATCCTCACAATACCGCAGAGTCTAGACTC1870.15998494259363824No Hit
CTGTCCAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTG1740.1488629947127971No Hit
CTCCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCG1740.1488629947127971No Hit
GTATTATGAATGGAAATCAGAGGAAAAAGAGATTTATAAGAGTCTTTTAA1710.14629639135567987No Hit
ATTTTACACAGCCCCTATTCAAGATGGAGTTGCTCTAGTTCAACTGCCTC1700.1454408569033075No Hit
GCTCTGTATTATGAATGGAAATCAGAGGAAAAAGAGATTTATAAGAGTCT1630.13945211573670072No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTCTGTATGCGTGTATT1600.13688551237958355No Hit
CCTTAGAATCTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCAA1520.13004123676060433No Hit
CTCCAAAAGACACCAAATACTCTATGACGGTTTCTCTTCCAAAAGTGAGA1520.13004123676060433No Hit
CCTACAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA1500.12833016785585954No Hit
TCTACTCTCCTCCCCCGACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA1480.12661909895111476No Hit
CAATCACATAACCCATGAAATTTAGGGAATATCCCCATCTTTTTGTTTTG1450.12405249559399756No Hit
GTCCATGTACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA1450.12405249559399756No Hit
CTCCACCCCAAAAGGCCTCCGTGTGGGGGAGTGAACCCCGGCCCGAATGC1450.12405249559399756No Hit
GCTCAGCTCTGTATTATGAATGGAAATCAGAGGAAAAAGAGATTTATAAG1440.12319696114162516No Hit
GGGAGGGAGCCCTCAGGCTCAAGGCATGCTAAAAACAATGCCAGCAGATC1440.12319696114162516No Hit
GGTCAGTAATCTACTCTCCTCCCCCGACTCCTCCCAGTCTTTAAACAAAC1420.12148589223688039No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT1410.120630357784508No Hit
GCCTTAGAATCTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCA1390.11891928887976318No Hit
CTATTTACATACCCTGTGGAAGGCGGGCATCTTATATAAAAGAGAAACTG1380.11806375442739078No Hit
CCTTTATACCGCTGTTACTAATTTTCTTTTGTCTTTGGGTATACATTTAA1370.11720821997501839No Hit
GTTCCTGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA1360.116352685522646No Hit
GCTTTACTCTTCTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCAT1330.11378608216552882No Hit
CTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGAT1300.11121947880841161No Hit
TAGTGAGACCTTGTATCTACAAAAAATAAAATTGTCAGAGGCAGTTGAAC1290.11036394435603922No Hit
TTCTTGGACAGTCCCTCTCGAATGGGGGAAGAATCATTCCACCACCAATC1270.10865287545129443No Hit
CTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATTCATTTGCACC1260.10779734099892202No Hit
ATACTAATATGGGCTTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCT1250.10694180654654963No Hit
CTATTCCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTCCTGATATT1250.10694180654654963No Hit
CTCCCACTCCTACCTTGTTGGCTTCTGTCCAGTGGTCTTTGTTGGGATTG1250.10694180654654963No Hit
GCTTAGGGATCTATCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACG1240.10608627209417723No Hit
TATCAGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCAT1240.10608627209417723No Hit
GTATGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATTCTCTGG1230.10523073764180484No Hit
GGGGTGGAGCCCTCAGGCTCAAGGCATGCTAAAAACAATGCCAGCAGATC1220.10437520318943244No Hit
ACCCATCTCGGGGTCGCTTGGGGATCTATCGTCCCCTCCTCCGTCTGCCG1210.10351966873706005No Hit
CTCTTCTACTATTCCTGTCTTTAATCCTAACTGGAAAACTCCATCTTTTC1210.10351966873706005No Hit
TTCTCTGGATAATAAGGTTTTATACCTTTCTCTAGGGGCAAATATTTCGT1190.10180859983231524No Hit
GTCCAAGAAAGCCCCATGATGTAGCTCTTGTTCCCAAGAATAAGGCGTCC1180.10095306537994285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)