FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605399
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73721.2177093123708496No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19090.3153292291530049No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17290.2855967717158436No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG15190.2509089047058221No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13000.21473441482394257No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG11970.19772084195712247No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11700.1932609733415483No Hit
TTCTAAAACATTGCTTGATTTTTAGTACAATATGTTCCTGCGGTAAAGTA10160.16782320420086588No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9420.1555998605878107No Hit
GTTTGTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGTCT9070.14981854941947376No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAA8930.147506024952139No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8900.14701048399485298No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT8810.14552386112299492No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG8650.14288097601746946No Hit
GGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGTAAATGAATG8630.1425506153792788No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8420.13908182867827665No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG8400.13875146804008595No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7920.13082281272350962No Hit
TCTTATGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG7890.13032727176622358No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7880.13016209144712826No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7710.12735402602250748No Hit
AACTCAGACAACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAAAGATAC7680.12685848506522146No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATA7640.12619776378884007No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7530.12438078027879133No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7390.12206825581145657No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7190.11876464942954977No Hit
GTATCTTTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGTCTGAGTT7130.11777356751497772No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA6950.11480032177126161No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6920.11430478081397558No Hit
GAAAAGATACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGC6890.11380923985668955No Hit
ATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG6850.1131485185803082No Hit
GATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTGGAGGAC6700.1106708137938781No Hit
AACTAAGACAACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGACAAAC6670.11017527283659206No Hit
CTTTTACACAATGTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATA6510.10753238773106662No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6320.10439396166825514No Hit
TTAGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGA6230.10290733879639707No Hit
GTATCTTTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGTCT6180.10208143720092039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)