FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605399
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145312.4002352167743917No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18580.30690503287914256No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15900.2626367073615913No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG13100.21638621801489594No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12420.2051539563164128No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG11020.18202871164306514No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10480.17310897441191678No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9590.15840792601243145No Hit
GTTTGTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGTCT9140.15097481165314114No Hit
TTCTAAAACATTGCTTGATTTTTAGTACAATATGTTCCTGCGGTAAAGTA8880.1466801233566623No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8680.14337651697475548No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8550.14122917282651606No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAA8270.13660412389184654No Hit
GGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGTAAATGAATG8270.13660412389184654No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG8060.1331353371908444No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7780.12851028825617486No Hit
TCTTATGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG7680.12685848506522146No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG7620.1258674031506494No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7500.12388523932150532No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7410.12239861644964725No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATA7400.12223343613055192No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7340.12124235421597988No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7170.11843428879135909No Hit
AACTCAGACAACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAAAGATAC6980.11529586272854761No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT6910.11413960049488023No Hit
GTATCTTTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGTCTGAGTT6830.11281815794211751No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6730.1111663547511641No Hit
CTTTTACACAATGTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATA6640.10967973187930603No Hit
ATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG6630.10951455156021071No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6140.10142071592453901No Hit
GAAAAGATACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGC6070.10026445369087165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)