FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369648
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACA14180.3836081894126304No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10760.2910877375232654No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTATGAGTGCAGTA9670.26160022507899405No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9090.24590962212699652No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9040.24455698394147946No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGGTACG8510.2302190191749989No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6730.18206509977059257No Hit
ATCTAGAAGATCTCGTACCGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA6710.18152404449638576No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6700.18125351685928234No Hit
GCTTTAGGGCATGGACCTCGACCCTTATAAAGAATTTGGAGCTTCTGTGG6420.17367874302038697No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACCGAAGGAAA6260.16935030082673247No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6250.16907977318962905No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6200.16772713500411202No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCCTGTATT6160.1666450244556984No Hit
CATTTAAACCCCAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT6150.16637449681859498No Hit
CTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT6000.1623165822620439No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC5660.15311864260052807No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5540.1498723109552872No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT5480.14824914513266674No Hit
CTCTATGACTACTTCTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA5470.14797861749556335No Hit
CTCTAAGGCGTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5390.14581439639873608No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5360.14500281348742589No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5190.14040384365666797No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5170.13986278838246116No Hit
ACCTTATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG5100.13796909492273732No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4990.13499329091459983No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACCTCGA4990.13499329091459983No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG4960.13418170800328963No Hit
ACTACATGTTCCGGATAATAAGGTTTAATACCCTTATCTAAGGGCAAATA4890.13228801454356576No Hit
AATACACGTCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4780.1293122105354283No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4770.1290416828983249No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4760.1287711552612215No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4740.12823009998701465No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGACGCCTTAGAGTCTCCCGA4660.12606587889018742No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4630.12525429597887722No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG4530.12254901960784313No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4470.12092585378522269No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4440.12011427087391248No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGT4420.11957321559970567No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4410.11930268796260227No Hit
ATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACCGAAGGAAAGAAGT4300.11632688395446479No Hit
TCTAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTT4250.11497424576894776No Hit
ATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAG4220.11416266285763754No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACCTCGACCCTTAT4190.11335107994632733No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGACCTA4160.11253949703501709No Hit
GTTTAATACCCTTATCTAAGGGCAAATATTTGGTAACATTTGGATAAAAC4140.1119984417608103No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTAGATGATGGATCTTC4130.11172791412370688No Hit
CCCTTATCTAAGGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4120.11145738648660347No Hit
ACATAGAGGTTCCTTGAGCAGTTGTCGTGCAGGTTCTGCATGGTCCCGCG4100.11091633121239666No Hit
CACTCACAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGG4090.11064580357529326No Hit
ATCCAAATGTTACCAAATATTTGCCCTTAGATAAGGGTATTAAACCTTAT4080.11037527593818984No Hit
ACCAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGC4050.10956369302687963No Hit
GTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG4010.10848158247846601No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTT3980.10766999956715578No Hit
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAAA3950.10685841665584557No Hit
TCCTAGTACAAAGACCTTTAACCTTATCTCCTCCCCCAACTCCTCCCAGT3940.10658788901874215No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG3920.10604683374453536No Hit
CCTCTATGTATCCCTCATGTTGCTGTACCAAACCTTCGGAAGGAAATTGC3920.10604683374453536No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT3910.10577630610743194No Hit
GTTCATGTATTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGA3850.1041531402848115No Hit
GAATACAGGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA3820.10334155737350129No Hit
CCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3820.10334155737350129No Hit
ACGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3770.10198891918798425No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3750.10144786391377743No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG3710.1003657533653638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)