FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484161
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAA13420.27718052466018533No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12150.25094958082125574No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT12010.2480579807130273No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10810.2232728369282119No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10500.2168700081171346No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC10040.20736903633295534No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCACACCCGTG9320.19249795006206613No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9290.19187832146744577No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8420.17390909222345458No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAATAGTCCCCCATG8290.1712240349800996No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8090.16709317768263038No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTTA7190.14850431984401882No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7070.14602580546553728No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7060.14581926260066383No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC6510.13445940503262344No Hit
TCCCAATAGTCCCCCATGGGGAGGGACGAACCCTGGCCCGAATGCTCCCA6310.1303285477351542No Hit
GCCTAGCAGCCATGGGAATGACGTATACTTGCGGGAGAGCACGACAGAAT6120.12640423330255846No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6070.12537151897819115No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6000.1239257189240769No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTAG5780.11938177589686076No Hit
CCCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA5710.11793597584274652No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5620.11607709005888536No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA5590.115457461464265No Hit
CTATTAGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTA5480.11318548995065691No Hit
ATATGCAGGAGGAGTGCGAATCCACACCCCAAAAGACACCAAATATTCAA5360.11070697557217536No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATCCCAGGATCATCAACAACC5340.11029388984242845No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5230.10802191832882037No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5030.10389106103135114No Hit
GTATCGGGAGGCCTTAGAGTCTTCGGAACATTGTACACCTCACCATACGG5010.10347797530160421No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTC5000.10327143243673076No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5000.10327143243673076No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4860.10037983232850228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)