FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484161
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12050.2488841521725211No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAA11480.23711120887473383No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT10470.2162503795225142No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8840.18258389254813998No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8800.18175772108864613No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCACACCCGTG8610.17783340665605035No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8110.1675062634123773No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7170.1480912341142719No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6690.13817717660034576No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6580.13590520508673767No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAATAGTCCCCCATG6570.13569866222186422No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6360.13136126205952153No Hit
CCCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA6140.1268173190323054No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5650.11669671865350574No Hit
TCCCAATAGTCCCCCATGGGGAGGGACGAACCCTGGCCCGAATGCTCCCA5480.11318548995065691No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5300.1094677183829346No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC5110.10554340395033883No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTTA4910.1014125466528696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)