FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455413
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28250.6203160647588014No Hit
GTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAATTCTTTGACATACT23230.510086449003432No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG23060.5063535735694853No Hit
GGAACATATCGTACAAAAAATCAAACAATGTTTTCGGAAACTGCCTGTAA21370.46924440013789676No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC21210.46573110561182923No Hit
GCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG18530.4068834223001978No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA18090.3972218623535121No Hit
GCCTAGAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT18080.3970022814456329No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA17870.3923910823801692No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG17620.3869015596831887No Hit
CACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTAACTTCAT17200.37767916155226133No Hit
CTTTATACCTCTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAAA14840.325858067292765No Hit
CCTTTATACCTCTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAA14240.3126832128200117No Hit
GGTATACACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTAA14070.3089503373860649No Hit
GTATACATGCATACAAAGGCATTAATGCTGGATAACCACATTGTGTAAAA13430.2948971592817948No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGTACGATA12570.2760132012041817No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12140.26657122216537515No Hit
CCTTTTACACAATGTGGTTATCCAGCATTAATGCCTTTGTATGCATGTAT11910.26152086128415303No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGT11710.2571292431265686No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11500.252518044061105No Hit
CTCCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAG11490.2522984631532258No Hit
AGACTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAATTTTCTT11290.24790684499564133No Hit
AGTTAAGGCAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAGTGTATA11290.24790684499564133No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATCGTACAAAAA11140.244613131377453No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGGGCAAC11100.24373480774593612No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGTACGATATGTT11090.2435152268380569No Hit
CTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTAACTTCATGG10850.23824528504895556No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGTACGAT10840.23802570414107635No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC10820.2375865423253179No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG10750.23604947597016335No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT10340.22704665874711524No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10320.22660749693135682No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC10110.22199629786589314No Hit
GTATAAAGGGATTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACATC10040.2204592315107386No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG9750.21409138518224116No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG9600.21079767156405282No Hit
GTACAACATCTTGAATCCCTTTATACCTCTATCACTAATTTTCTTTTGTC9400.20640605340646845No Hit
GGATAACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAAT9120.20025778798585023No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC8910.19564658892038655No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT8800.19323119893371513No Hit
CTGGATAACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACA8490.18642419078945924No Hit
GTCTTTGGGTATACACTTAAACCCTAATAAAACCAAACGTTGGGGCTACT8320.18269131535551247No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8230.18071508718459947No Hit
GGTATAAAGGGATTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACAT8170.17939760173732414No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGAC7860.17259059359306828No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7860.17259059359306828No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7820.1717122699615514No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTG7360.1616115481991072No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT7270.1596353200281942No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT7240.15897657730455653No Hit
CAATTACATATCCCATGAAGTTAAGGCAGTAGCCCCAACGTTTGGTTTTA7240.15897657730455653No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACA7170.15743951094940198No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATCGTACA7150.15700034913364352No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT7090.1556828636863682No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGTAAGATTCCTATGGGAG7040.1545849591469721No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7000.1537066355154552No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6930.15216956916030064No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTG6840.15019334098938766No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT6840.15019334098938766No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAA6820.1497541791736292No Hit
CTACCGAGGTATGTTGCCCGTTTGTCCTCTGCTTCCAGGAACATCAACTA6790.14909543644999157No Hit
TACATATCCCATGAAGTTAAGGCAGTAGCCCCAACGTTTGGTTTTATTAG6710.14733878918695778No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6690.14689962737119933No Hit
CCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6590.1447038182924071No Hit
GGCCAAGACTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAATT6580.1444842373845279No Hit
CCTCTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAAACCCTAAT6560.14404507556876944No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6490.1425080092136149No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6470.14206884739785647No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTATACC6470.14206884739785647No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTAGCTTGTA6370.13987303831906422No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6360.139653457411185No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTAG6360.139653457411185No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATA6330.13899471468754734No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGCAGTAGCCCCA6280.13789681014815125No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6270.137677229240272No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6160.13526183925360058No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6070.13328561108268758No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6050.13284644926692912No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCT5970.13108980200389536No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5930.13021147837237848No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5910.12977231655662003No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGACC5870.12889399292510315No Hit
CATGCATACAAAGGCATTAATGCTGGATAACCACATTGTGTAAAAGGAGC5840.12823525020146548No Hit
GTGTATACCCAAAGACAAAAGAAAATTAGTGATAGAGGTATAAAGGGATT5790.12713734566206938No Hit
ATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG5770.12669818384631093No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5760.12647860293843172No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT5670.12450237476751873No Hit
CTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAAACCCTAATAAA5580.12252614659660573No Hit
TAACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAATTCT5430.1192324329784174No Hit
GGGTATACACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTA5430.1192324329784174No Hit
CACTTGGCACAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT5420.11901285207053817No Hit
CTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAATTTTCTTTTG5380.11813452843902128No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTAGGCTTT5370.11791494753114207No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5350.11747578571538363No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5280.11593871936022906No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5270.11571913845234985No Hit
GGTTCCACGCATGCGCTGATGGCCGATGGCCAAGCCCCAGCCAGTGGGGG5270.11571913845234985No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT5190.11396249118931608No Hit
ATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTT5160.11330374846567841No Hit
GCACAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTT5090.11176668211052385No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC4990.10957087303173163No Hit
ATCTTGGGCTTTCGTAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4930.1082533875844563No Hit
GGGCCAAGACTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAAT4890.10737506395293941No Hit
GTCTTAGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4880.10715548304506019No Hit
GCCCAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTTT4860.10671632122930175No Hit
ACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAATTCTTT4810.10561841668990564No Hit
CAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG4770.10474009305838874No Hit
CTGCAAATTTTGGCCAGGACACGTGGGTGCTCCCCCTAGAAAATTGAGAG4760.10452051215050953No Hit
GTTTAAGTGTATACCCAAAGACAAAAGAAAATTAGTGATAGAGGTATAAA4660.10232470307171733No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4620.10144637944020043No Hit
GGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCC4600.101007217624442No Hit
GCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGG4590.10078763671656278No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4580.10056805580868355No Hit
CTTCTAGGCTTTGCCGCTCCTTTTACACAATGTGGTTATCCAGCATTAAT4560.10012889399292511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)