FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455413
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28080.6165831893248546No Hit
GGAACATATCGTACAAAAAATCAAACAATGTTTTCGGAAACTGCCTGTAA21560.47341643738760203No Hit
GTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAATTCTTTGACATACT21180.4650723628881916No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG21060.4624373919936409No Hit
GCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG20270.44509050027118247No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG19290.4235715712990187No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA19040.4180820486020381No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA17860.39217150147229No Hit
GCCTAGAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT17630.3871211405910679No Hit
CACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTAACTTCAT16970.37262880067103926No Hit
CTTTATACCTCTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAAA15070.33090842817398713No Hit
GTATACATGCATACAAAGGCATTAATGCTGGATAACCACATTGTGTAAAA13640.2995083583472584No Hit
CCTTTATACCTCTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAA13620.2990691965315No Hit
GGTATACACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTAA13600.29863003471574157No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC13500.2964342256369493No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGTACGATA12120.26613206034961673No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATCGTACAAAAA11940.2621796040077907No Hit
CTCCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAG11900.2613012803762738No Hit
CCTTTTACACAATGTGGTTATCCAGCATTAATGCCTTTGTATGCATGTAT11660.25603133858717253No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11380.24988307316655434No Hit
CTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTAACTTCATGG11300.24812642590352055No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGT11240.2468089404562452No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC11170.24527187410109066No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGTACGATATGTT11080.24329564593017766No Hit
AGACTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAATTTTCTT10990.2413194177592647No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10910.2395627704962309No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC10380.22792498237863215No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTTTGTACGAT10360.2274858205628737No Hit
AGTTAAGGCAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAGTGTATA10340.22704665874711524No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG10310.22638791602347758No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG10170.22331378331316848No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10160.22309420240528927No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGGGCAAC9960.2187025842477048No Hit
GTATAAAGGGATTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACATC9870.21672635607679183No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC9610.21101725247193204No Hit
GTACAACATCTTGAATCCCTTTATACCTCTATCACTAATTTTCTTTTGTC9360.20552772977495154No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT9220.20245359706464244No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG8710.19125497076280212No Hit
GGATAACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAAT8620.18927874259188912No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATCGTACA8210.18027592536884102No Hit
GTCTTTGGGTATACACTTAAACCCTAATAAAACCAAACGTTGGGGCTACT8190.1798367635530826No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8100.1778605353821696No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7830.1719318508694306No Hit
GGTATAAAGGGATTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACAT7640.1677598136197254No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAG7470.16402693818577863No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7390.16227029092274484No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTG7310.16051364365971107No Hit
CCTCTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAAACCCTAAT7240.15897657730455653No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7240.15897657730455653No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7230.15875699639667729No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTG7210.15831783458091886No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATA7100.1559024445942474No Hit
CTGGATAACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACA7090.1556828636863682No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT7080.15546328277848898No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGTAAGATTCCTATGGGAG7010.15392621642333443No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCT7010.15392621642333443No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGAC6920.15194998825242143No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT6920.15194998825242143No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT6900.15151082643666297No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAA6890.15129124552878376No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTAG6880.15107166462090454No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT6830.14997376008150845No Hit
CTACCGAGGTATGTTGCCCGTTTGTCCTCTGCTTCCAGGAACATCAACTA6650.14602130373968245No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6600.14492339920028635No Hit
TACATATCCCATGAAGTTAAGGCAGTAGCCCCAACGTTTGGTTTTATTAG6600.14492339920028635No Hit
GGCCAAGACTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAATT6560.14404507556876944No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6480.14228842830573568No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6460.14184926648997723No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACA6450.141629685582098No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6430.14119052376633956No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6400.1405317810427019No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT6370.13987303831906422No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGACC6300.13833597196390968No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAATCCCTTTATACC6300.13833597196390968No Hit
CAATTACATATCCCATGAAGTTAAGGCAGTAGCCCCAACGTTTGGTTTTA6240.13701848651663434No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTAGCTTGTA6150.13504225834572137No Hit
CCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5870.12889399292510315No Hit
GGGTATACACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTGCCTTA5860.1286744120172239No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5860.1286744120172239No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGCAGTAGCCCCA5850.1284548311093447No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5690.12494153658327717No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5570.1223065656887265No Hit
GTGTATACCCAAAGACAAAAGAAAATTAGTGATAGAGGTATAAAGGGATT5560.12208698478084729No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5490.12054991842569272No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5480.12033033751781351No Hit
CATGCATACAAAGGCATTAATGCTGGATAACCACATTGTGTAAAAGGAGC5450.11967159479417584No Hit
ATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG5420.11901285207053817No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTAGGCTTT5420.11901285207053817No Hit
CTTCTAGGCTTTGCCGCTCCTTTTACACAATGTGGTTATCCAGCATTAAT5350.11747578571538363No Hit
CACTTGGCACAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT5300.1163778811759875No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5290.11615830026810829No Hit
GCACAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTT5290.11615830026810829No Hit
CTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAATTTTCTTTTG5230.11484081482083296No Hit
CTATCACTAATTTTCTTTTGTCTTTGGGTATACACTTAAACCCTAATAAA5220.11462123391295373No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5190.11396249118931608No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5050.11088835847900697No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT5030.11044919666324853No Hit
GGGCCAAGACTGTACAACATCTTGAATCCCTTTATACCTCTATCACTAAT4950.10869254940021475No Hit
GTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGA4910.10781422576869786No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4900.10759464486081863No Hit
AATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCTTGTCCTCCAAT4860.10671632122930175No Hit
ATCTTGGGCTTTCGTAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4840.1062771594135433No Hit
ACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAATTCTTT4790.1051792548741472No Hit
GTCTTAGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4760.10452051215050953No Hit
TAACCACATTGTGTAAAAGGAGCGGCAAAGCCTAGAAGACCCACAATTCT4740.1040813503347511No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4720.10364218851899266No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCACATCGTCAATCTTCTCG4710.10342260761111344No Hit
GGTTCCACGCATGCGCTGATGGCCGATGGCCAAGCCCCAGCCAGTGGGGG4610.10122679853232122No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4600.101007217624442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)