FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417386
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT29210.69983181036259No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24680.5912991810937598No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTATTTGGTAACATT17180.41160939753609366No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT15250.3653692265672543No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA14670.35147321663879477No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGACTA13320.31912905559841487No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGTTCTGG12860.3081080822068781No Hit
ATCTAAGGGGAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA12810.3069101503164936No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA12520.29996214535226384No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG12350.29588917692495675No Hit
GCTACGTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTAT12220.2927745540099572No Hit
ATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA11300.27073260722688347No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA11240.2692950889584222No Hit
CAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT11230.2690555025803453No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC11110.2661804660434226No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA10620.2544407335176551No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10490.2513261106026556No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA10150.24318017374804138No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA10110.24222182823573382No Hit
CATTATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAA9650.23120085484419697No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8700.2084401489268926No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8500.20364842136535488No Hit
CTATTATCCAGAACATGTAGTTAATCATTACTTCCAGACGAGACATTATT8470.20292966223112419No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8440.20221090309689352No Hit
CTCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG8440.20221090309689352No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAATTTCAT8370.20053379845035532No Hit
GTTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTATTTGGTA8270.19813793466958643No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8090.19382537986420245No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA7980.19118992970535667No Hit
GCTATGTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTAT7880.1887940659245878No Hit
GACATATCCCATGAAATTAAGGGAATACCCCCATCTTTTTGTTTTGTGAG7810.18711696127804958No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG7730.18520027025343447No Hit
AATCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7530.1804085426918967No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA7530.1804085426918967No Hit
ACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATG7520.18016895631381982No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC7330.17561681513035896No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGTTCT7180.17202301945920564No Hit
ACCAAATACTTCCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAACA7070.16938756930035986No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAACATGTAGTTAATCA6900.16531460087305277No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6630.15884576866497677No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACGATTCGTTGACATACT6590.15788742315266924No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6520.156210318506131No Hit
CCTTTATCTAAGGGGAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG6520.156210318506131No Hit
AATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAA6480.15525197299382346No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6140.14710603613920925No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT6120.14662686338305547No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5860.1403976175530564No Hit
CAAGCAGATGAGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAACGCC5810.13919968566267196No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT5580.13368919896690354No Hit
AGATAAAGGAATCAAACCCTATTATCCAGAACATGTAGTTAATCATTACT5540.13273085345459598No Hit
AAATTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTAT5420.12985581691767334No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA5350.12817871227113511No Hit
AGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAAT5330.12769953951498134No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC5310.12722036675882756No Hit
CTACATGTTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTAT5260.12602243486844314No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC5180.12410574384382801No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5100.12218905281921291No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5070.12147029368498226No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4950.11859525714805957No Hit
ATCCAGAACATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT4910.11763691163575205No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4890.11715773887959827No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4880.11691815250152136No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4860.1164389797453676No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4790.11476187509882939No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT4770.11428270234267561No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4760.1140431159645987No Hit
CCTAACAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGAT4740.11356394320844493No Hit
TGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4690.1123660113180605No Hit
AATTAATTATGCCTGCTAGATTTTATCCCAATGTTACCAAATACTTCCCC4640.11116807942767605No Hit
TTGTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAAC4590.10997014753729162No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCAG4570.10949097478113784No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACGATTCG4560.10925138840306096No Hit
GACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGTTACCAAATAC4560.10925138840306096No Hit
ACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAGAGCT4450.10661593824421518No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCGATGACATATCCCATGAA4390.10517841997575386No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATCGTGGGTCT4380.10493883359767697No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4380.10493883359767697No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4350.1042200744634463No Hit
ATCCCAATGTTACCAAATACTTCCCCTTAGATAAAGGAATCAAACCCTAT4320.10350131532921565No Hit
CATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCA4320.10350131532921565No Hit
CCCTAACAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA4300.10302214257306187No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGTTCTGGATAATAGG4280.1025429698169081No Hit
ATGTAAGACTGTTGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC4260.10206379706075432No Hit
ATTTTATCCCAATGTTACCAAATACTTCCCCTTAGATAAAGGAATCAAAC4260.10206379706075432No Hit
ACCCCTAACAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTA4240.10158462430460054No Hit
CCTCCTTCATATAGACCTCAAAATGCCCCTATCTTATCAACACTTCCGGA4220.10110545154844676No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4180.10014710603613922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)