FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417386
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT25450.6097473322056801No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24540.5879449718006833No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTATTTGGTAACATT18070.4329325851849367No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT16980.4068176699745559No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGTTCTGG13870.33230630639264375No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA13710.3284729243434135No Hit
GCTACGTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTAT13320.31912905559841487No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGACTA12390.2968475224372643No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAG12180.29181620849764967No Hit
ATCTAAGGGGAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA12180.29181620849764967No Hit
ATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA11550.27672226667880573No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA11240.2692950889584222No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA10940.26210749761611557No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA10280.24629479666304094No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA10260.24581562390688713No Hit
CAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT10240.24533645115073338No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC10200.24437810563842582No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10120.2424614146138107No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA9050.21682567215958368No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA8960.21466939475689167No Hit
CATTATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAA8710.2086797353049695No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC8670.20772138979266194No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8580.20556511238996994No Hit
CTCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG8430.20197131671881663No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8380.20077338482843218No Hit
GCTATGTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTAT8270.19813793466958643No Hit
GTTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTATTTGGTA8150.19526289813266376No Hit
CTATTATCCAGAACATGTAGTTAATCATTACTTCCAGACGAGACATTATT8050.1928670343518949No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA7990.19142951608343356No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7770.18615861576574202No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAATTTCAT7720.18496068387535758No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACGATTCGTTGACATACT7280.1744188832399745No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGTTCT7260.17393971048382073No Hit
CCTTTATCTAAGGGGAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG7190.1722626058372825No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7050.16890839654420609No Hit
ACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATG6890.16507501449497586No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG6850.16411666898266833No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6790.162679150714207No Hit
AATCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6760.16196039157997633No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAACATGTAGTTAATCA6730.16124163244574566No Hit
GACATATCCCATGAAATTAAGGGAATACCCCCATCTTTTTGTTTTGTGAG6630.15884576866497677No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6570.15740825039651546No Hit
ACCAAATACTTCCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAACA6550.15692907764036168No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT6440.1542936274815159No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA6280.1504602454322857No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6110.1463872770049786No Hit
CTACATGTTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTAT5710.1368038218819031No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5480.13129333518613465No Hit
AATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAA5460.13081416242998087No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5170.12386615746575114No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCAG5170.12386615746575114No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT5100.12218905281921291No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5060.12123070730690536No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC5020.1202723617945978No Hit
AGATAAAGGAATCAAACCCTATTATCCAGAACATGTAGTTAATCATTACT4990.11955360266036714No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4990.11955360266036714No Hit
CCTAACAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGAT4980.11931401628229026No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4920.11787649801382892No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCGATGACATATCCCATGAA4910.11763691163575205No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4850.11619939336729071No Hit
ATCCAGAACATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT4820.11548063423306004No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT4780.11452228872075249No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4730.11332435683036805No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATCGTGGGTCT4700.1126055976961374No Hit
GTAATGATTAACTACATGTTCTGGATAATAGGGTTTGATTCCTTTATCTA4690.1123660113180605No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4630.11092849304959918No Hit
AGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAAT4530.1085326292688303No Hit
CCCTAACAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA4520.10829304289075341No Hit
TTGTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAAC4500.10781387013459962No Hit
GTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGACTAA4450.10661593824421518No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4430.1061367654880614No Hit
ATTTTATCCCAATGTTACCAAATACTTCCCCTTAGATAAAGGAATCAAAC4370.10469924721960008No Hit
ATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4360.1044596608415232No Hit
AAATTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTAT4340.10398048808536942No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4330.10374090170729253No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT4320.10350131532921565No Hit
TGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4300.10302214257306187No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4300.10302214257306187No Hit
ATTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4290.10278255619498497No Hit
CAAGCAGATGAGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAACGCC4240.10158462430460054No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACGATTCG4230.10134503792652366No Hit
ACCCCTAACAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTA4220.10110545154844676No Hit
TTTGAGGTCTATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACACC4210.10086586517036988No Hit
GACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGTTACCAAATAC4210.10086586517036988No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAACACCTTGAGTCCCTTTATGCC4200.10062627879229298No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGTTCTGGATAATAGG4190.10038669241421609No Hit
GTATAAAGAATTTGGAGCTTCTGCAGAATTACTCTCTTTTTTGCCTTCTG4190.10038669241421609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)