FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496543
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACCTT20730.41748650167256407No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18770.3780135859331417No Hit
CATTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14880.29967193173602286No Hit
GTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTAA14360.28919952551944145No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT13320.2682547130862785No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT11760.23683749443653418No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGA11720.2360319247275664No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC11700.23562913987308248No Hit
ATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAA11610.23381660802790494No Hit
GTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC11220.22596230336546885No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10960.22072610025717812No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTTCAGACGAGACATTATT10930.22012192297545227No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA9570.19273255287054694No Hit
GTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACATGCTCGGG9430.18991305888915966No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACA8930.1798434375270621No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT8730.17581558898222308No Hit
CTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT8700.17521141170049725No Hit
GGTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTA8600.17319748742807775No Hit
GGGATTTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGA8530.17178774043738407No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8460.1703779934466904No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT8330.16775989189254506No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8310.16735710703806114No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAACAAGTTGGTGAGTGAC8280.1667529297563353No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8250.16614875247460945No Hit
GGTGTGAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTA8170.16453761305667386No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACCACCAATTACATATCCCATGAA8130.16373204334770602No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGAA8090.16292647363873825No Hit
AGTCTGTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTT7960.16030837208459287No Hit
CAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT7960.16030837208459287No Hit
CAACCACCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTT7860.15829444781217336No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA7690.1548707765490602No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGG7520.15144710528594704No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCGGG7500.15104432043146315No Hit
CAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATATTTGT7400.14903039615904362No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA7300.14701647188662412No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT7210.14520394004144657No Hit
ACCCCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTA6870.13835659751522023No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC6870.13835659751522023No Hit
ATGGAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA6830.13755102780625245No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGT6770.13634267324280072No Hit
GCTCACACCTATCAACAATGTTTTCCCGCAAATGAATGTCAGGAAAAGAA6750.13593988838831683No Hit
CCCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGA6630.1335231792614134No Hit
GATTAACTACATGCTCGGGATAATAGGGTTTGATTCCTTTATCTAAGGGC6590.13271760955244563No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6400.12889115343484853No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6260.12607165945346124No Hit
TTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGA6230.12546748217173537No Hit
GTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTTTTGTC6220.1252660897444934No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6190.12466191246276759No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT6180.12446052003552563No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6170.12425912760828367No Hit
GTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCA6110.12305077304483197No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6100.12284938061759001No Hit
GCTTACACCTATCAACAATGTTTTCCCGCAAATGAATGTCAGGAAAAGAA6070.12224520333586415No Hit
ATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTA5930.11942570935447686No Hit
CAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATATTTGT5880.11841874721826709No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC5860.1180159623637832No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5840.1176131775092993No Hit
ATGTGGTATTGGGGGCAAAGTCTGTACAACATCTTGAGTCCATTTATACC5760.1160020380913637No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT5730.11539786080963783No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5680.11439089867342808No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5630.11338393653721832No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA5610.11298115168273443No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG5560.11197418954652467No Hit
ATATGGAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5530.11137001226479881No Hit
GTTGATAGGTGTGAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAG5460.10996026527410516No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTG5460.10996026527410516No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT5450.1097588728468632No Hit
TTTCCAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAAT5450.1097588728468632No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG5420.10915469556513736No Hit
CCAAAGAGTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACAT5380.10834912585616956No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5380.10834912585616956No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCAAAGTCTGTACAACATCTTG5360.10794634100168565No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5350.1077449485744437No Hit
AGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCATTACT5310.1069393788654759No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5300.10673798643823394No Hit
ATAATGTCTCGTCTGAAAGTAATGATTAACTACATGCTCGGGATAATAGG5290.106536594010992No Hit
ATCCCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5220.10512684702029834No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5200.10472406216581445No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT5140.10351570760236274No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA5100.10271013789339495No Hit
ATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTG5080.10230735303891102No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5050.10170317575718518No Hit
GGGTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACATGCTCG4980.10029342876649151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)