FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496543
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACCTT21490.4327923261429524No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18720.377006623796932No Hit
CATTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14860.299269146881539No Hit
GTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTAA14190.2857758542563283No Hit
GTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC13320.2682547130862785No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT13030.26241433269626196No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT12140.2444904066717283No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC11730.23623331715480833No Hit
ATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGGCATAA11470.2309971140465176No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTTCAGACGAGACATTATT10880.21911496083924253No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGA10720.2158926820033713No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10420.20985090918611277No Hit
GTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACATGCTCGGG10330.20803837734093522No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT9410.18951027403467574No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8910.1794406526725782No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT8850.1782322981091265No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA8770.1766211586911909No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8750.176218373836707No Hit
GGGATTTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGA8710.17541280412773919No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACA8510.17138495558290018No Hit
AGTCTGTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTT8280.1667529297563353No Hit
GGTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTA8190.16494039791115775No Hit
GGTGTGAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTA8000.16111394179356067No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA7980.16071115693907678No Hit
CTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT7900.15910001752114117No Hit
CAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT7890.1588986250938992No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCGGG7800.15708609324872166No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGAA7720.15547495383078605No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA7590.1528568522766407No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAACAAGTTGGTGAGTGAC7420.14943318101352754No Hit
CAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATATTTGT7410.14923178858628558No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC7350.14802343402283388No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA7330.14762064916834997No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACCACCAATTACATATCCCATGAA7250.14600950975041438No Hit
ATGGAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA7020.1413774839238495No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACCTTGGGATAAAATCTAGCAGG7000.1409746990693656No Hit
GTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCA6830.13755102780625245No Hit
GTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTTTTGTC6800.13694685052452657No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6750.13593988838831683No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGT6610.13312039440692952No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6590.13271760955244563No Hit
GATTAACTACATGCTCGGGATAATAGGGTTTGATTCCTTTATCTAAGGGC6570.13231482469796169No Hit
CAACCACCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTT6560.13211343227071976No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6540.13171064741623587No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC6480.13050229285278414No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT6360.12808558372588075No Hit
CAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATATTTGT6320.12728001401691294No Hit
TTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGA6310.12707862158967098No Hit
ACCCCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTA6240.12566887459897733No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6180.12446052003552563No Hit
GCTCACACCTATCAACAATGTTTTCCCGCAAATGAATGTCAGGAAAAGAA6150.12385634275379977No Hit
CCCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGA6140.12365495032655782No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6020.1212382411996544No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT6000.1208354563451705No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5930.11942570935447686No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT5880.11841874721826709No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA5760.1160020380913637No Hit
GCTTACACCTATCAACAATGTTTTCCCGCAAATGAATGTCAGGAAAAGAA5680.11439089867342808No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5590.11257836682825052No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA5550.11177279711928272No Hit
ATCCCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5410.1089533031378954No Hit
TTTCCAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAAT5400.10875191071065345No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5380.10834912585616956No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTC5340.10754355614720175No Hit
GTTGATAGGTGTGAGCAATTTGTAGGACCCCTAACAGTAAATGAAACCAG5320.10714077129271786No Hit
ATAATGTCTCGTCTGAAAGTAATGATTAACTACATGCTCGGGATAATAGG5230.10532823944754029No Hit
ATGTGGTATTGGGGGCAAAGTCTGTACAACATCTTGAGTCCATTTATACC5230.10532823944754029No Hit
GTTATATGGATGATGTGGTATTGGGGGCAAAGTCTGTACAACATCTTGAG5210.10492545459305638No Hit
AGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCATTACT5200.10472406216581445No Hit
ATTTGTAGGACCCCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTA5170.10411988488408859No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5170.10411988488408859No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5160.10391849245684663No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT5020.10109899847545933No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC5020.10109899847545933No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4990.10049482119373347No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4980.10029342876649151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)