FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427610
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32000.7483454549706509No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18330.4286616309253759No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAACCTTAT17620.41205771614321457No Hit
CTCTTACAGTGAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16510.3860994831739202No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9430.22052805126166367No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9200.21514931830406211No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8740.204391852388859No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8210.19199738079090758No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAAC8140.19036037510815929No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT8030.18778793760669768No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7370.17235331259792802No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7060.16510371600289986No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6930.16206356259208157No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT6810.15925726713594163No Hit
ATTTACAGGTAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT6510.15224152849559178No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6390.14943523303945183No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6390.14943523303945183No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6330.14803208531138187No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6120.14312106826313697No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5910.13821005121489208No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5850.13680690348682212No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5350.12511400575290568No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5300.12394471597951405No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTGAATGAAAAA5290.12371085802483572No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG5230.12230771029676575No Hit
GTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5080.11879984097659083No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4990.11669511938448586No Hit
ATGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC4880.11412268188302425No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4840.11318725006431093No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4830.1129533921096326No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTCAC4820.11271953415495428No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATACC4810.11248567620027596No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG4620.10804237506138771No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4510.10546993755992609No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4500.10523607960524776No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4450.10406678983185613No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4440.1038329318771778No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4400.10289750005846449No Hit
GGATAAAGGTATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4370.1021959261944295No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4320.10102663642103786No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4290.10032506255700288No Hit
CTTACAGTGAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4280.10009120460232454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)