FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427610
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31800.7436682958770843No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21800.5098103411987559No Hit
CTCTTACAGTGAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16380.3830593297631019No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAACCTTAT14810.34634363087860437No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9780.22871307967540516No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9370.2191249035335937No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9340.2184233296695587No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT9050.21164144898388718No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8420.19690839783915248No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAAC8180.19129580692687262No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8050.18825565351605436No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7590.17749818760085123No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7180.16791001145903978No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7050.16486985804822152No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT6670.15598325577044503No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6620.1548139659970534No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6560.15341081826898342No Hit
ATTTACAGGTAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT6130.1433549262178153No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5840.13657304553214378No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5750.13446832394003883No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5750.13446832394003883No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTGAATGAAAAA5730.13400060803068217No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5440.12721872734501063No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5420.12675101143565398No Hit
GTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5370.12558172166226234No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5120.11973527279530415No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5100.11926755688594748No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG5050.11809826711255582No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5040.1178644091578775No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5040.1178644091578775No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTCAC5040.1178644091578775No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATACC4950.11575968756577254No Hit
GTCTATTTACAGGTAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA4890.11435653983770258No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA4800.11225181824559761No Hit
ATGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC4690.10967938074413601No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4630.10827623301606605No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4630.10827623301606605No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4610.10780851710670937No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4430.10359907392249947No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4420.10336521596782115No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4400.10289750005846449No Hit
GTTCAGGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTA4390.10266364210378615No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG4370.1021959261944295No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4360.10196206823975117No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4320.10102663642103786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)