FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408166
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG30080.736955062401082No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26370.6460606713935017No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT22760.5576162639710314No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16010.39224237197610773No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11860.2905680531940436No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11200.2743981615323177No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10590.25945326166314686No Hit
GAATTCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA10240.2508783191152619No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9430.23103345207587106No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8820.2160885522067002No Hit
GACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACAT8760.2146185620556342No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8150.19967366218646335No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8150.19967366218646335No Hit
CAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGT8140.19942866382795235No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7950.1947736950162434No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7780.19060872292155642No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7520.1842387656002705No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6940.1700288608066326No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA6920.1695388640896106No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6630.16243391169279167No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT6430.1575339445225717No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6420.15728894616406072No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6240.15287897571086273No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6230.15263397735235174No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5990.1467540167480878No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5890.14430403316297782No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5820.14258904465340083No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5710.13989406270977983No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5700.13964906435126884No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5630.13793407584169187No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5540.13572909061509286No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5380.13180911687891692No Hit
CAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTCTAT5290.12960413165231793No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5090.12470416448209798No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5010.12274417761400998No Hit
CATATTGTTGACATCTATTAATAATGTCTTCCTGTAAATGAATGTTAGGA4990.122254180896988No Hit
GTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA4950.12127418746294401No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4910.1202941940289No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT4850.11882420387783403No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4830.11833420716081203No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4790.11735421372676803No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4770.11686421700974603No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4760.11661921865123503No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4760.11661921865123503No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG4750.11637422029272405No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4750.11637422029272405No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4730.11588422357570205No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA4640.11367923834910305No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4600.11269924491505907No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4600.11269924491505907No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4590.11245424655654808No Hit
ATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG4560.11171925148101508No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG4550.11147425312250409No Hit
CAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGT4510.11049425968846008No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4440.1087792711788831No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4430.1085342728203721No Hit
CATTTACAGGAAGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT4360.10681928431079513No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCT4350.10657428595228413No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA4340.10632928759377312No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4280.10485929744270713No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT4240.10387930400866313No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT4230.10363430565015215No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4220.10338930729164116No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG4150.10167431878206418No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4100.10044932698950917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)