FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434694
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAGAGAGTATGCAAATAATGTCTCGT23900.5498120516961357No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT18020.41454448416587303No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTAACCTT15030.3457604659829673No Hit
GTCATATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAA14350.33011727790123624No Hit
ATATGATCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA14190.3264365277643584No Hit
TGAAAACAGGAGACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGG13510.3107933396826273No Hit
CCTTTTTTCATTTACTGTAAGGGGTCCCACAAATTGCTTACATCTATCAA12830.29515015160089625No Hit
ATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT12190.28042715105338467No Hit
GACTTAAATTAATAATGCCTGCCAGATTTTATCCTAAGGTTACCAAATAT12120.2788168228685006No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12070.2776665884507263No Hit
ATCCTGAATGGCAAACTCCTTCTTTTCCCGACATTCATTTGCAGGAGGAC11990.2758262133822873No Hit
GTATACAGGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA11970.2753661196151776No Hit
TCCAGAGAGTATGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA11570.2661642442729828No Hit
GAAAACAGGAGACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGGT11140.2562722282801235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10950.25190133749258103No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10930.2514412437254713No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAAAGTCCAA10810.24868068112281283No Hit
GGATCATATTGTACAAAAAATCACACAATGTTTTAGGAAACTTCCTGTAA10770.24776049358859334No Hit
GACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGGTTACCAAATAT10690.2459201185201544No Hit
GAAAAAAGGAGACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGGT9810.225675992767326No Hit
GTATCTGACCCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG9190.21141308598692415No Hit
ATATAAGATACCCGCCTTCCAGAGAGTATGCAAATAATGTCTCGTCTGGA9110.2095727109184852No Hit
GGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCAG9090.20911261715137547No Hit
ATCCTAAGGTTACCAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT8480.19507975725452847No Hit
ATATAAAAGAGAGTCAACACATAGCGCCTCATTTTGCGGGTCACCATATT8330.19162905400120545No Hit
GTGTTGACTCTCTTTTATATAAGATACCCGCCTTCCAGAGAGTATGCAAA8300.19093891335054083No Hit
GTATGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG8230.18932858516565676No Hit
TTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8160.18771825698077269No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8090.1861079287958886No Hit
TTATATGCCTGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTA7260.1670140374608345No Hit
CCTGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7250.16678399057727966No Hit
GTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAA7210.16586380304306017No Hit
ATCACACAATGTTTTAGGAAACTTCCTGTAAACAGGCCTATTGATTGGAA7100.16333328732395663No Hit
CAGTCAATGAAAACAGGAGACTAAAATTAATAATGCCTGCCAGATTTTAT6970.16034267783774334No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6900.15873234965285926No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAATA6450.14838023989289018No Hit
CATTTACTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCC6440.14815019300933532No Hit
ACTCACATCTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6390.14699995859156098No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACACGAG6360.14630981794089634No Hit
GTATATGACCCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG5960.13710794259870163No Hit
GTCCCGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAGACGGGCAAC5770.1327370518111591No Hit
CTCCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGT5750.13227695804404938No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5730.13181686427693964No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTTTGTCAAAGAATTGTGGGTCT5720.1315868173933848No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5700.13112672362627503No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5680.1306666298591653No Hit
GCACGGGACCATGCAAGACCTGCACAACTCCTGCTCAAGGAACCTCTATG5520.12698587972228742No Hit
GAGTATGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCG5470.12583564530451308No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTCTTGCATGGTCCCGTG5310.12215489516763517No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5280.12146475451697056No Hit
AGCTTCCTGGGTGGGTAATAATTTACAAGATCCAGCGTCCAGGGATCTAG5210.11985442633208648No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA5200.11962437944853162No Hit
ATGTGTTGACTCTCTTTTATATAAGATACCCGCCTTCCAGAGAGTATGCA5130.11801405126364753No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5120.11778400438009265No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5110.1175539574965378No Hit
GGATATCCACATTGCGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT5040.11594362931165371No Hit
GATTAACTACATGCTCGGGATAATAAGGTTTGATCCCTTTATCTAAGGGC5040.11594362931165371No Hit
GATGAAAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTG5030.11571358242809884No Hit
CTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4910.11295301982544043No Hit
GATGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4910.11295301982544043No Hit
CCTGTTTTCATTTACTGTAAGGGGTCCCACAAATTGCTTACATCTATCAA4900.11272297294188556No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4800.11042250410633687No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA4750.10927226968856253No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCCTGTATACAAGCCAAACAG4730.10881217592145279No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4730.10881217592145279No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCATCAACCACC4720.10858212903789792No Hit
ATGAAAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGG4670.10743189462012358No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCGATTACATATCCCATGAA4650.10697180085301385No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4640.10674175396945898No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4630.1065117070859041No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4580.10536147266812976No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGTACGACAGAAT4520.10398119136680055No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4500.10352109759969083No Hit
ACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG4410.101450675647697No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4410.101450675647697No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4400.10122062876414215No Hit
CCCGATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4380.1007605349970324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)