FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434694
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAGAGAGTATGCAAATAATGTCTCGT23890.5495820048125808No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT15430.3549623413251621No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTAACCTT15100.34737079416785144No Hit
ATATGATCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA14220.32712666841502297No Hit
CCTTTTTTCATTTACTGTAAGGGGTCCCACAAATTGCTTACATCTATCAA13570.3121736209839565No Hit
GTCATATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAA13010.29929099550488386No Hit
TGAAAACAGGAGACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGG12140.27927691663561033No Hit
GTATACAGGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA12090.278126682217836No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11760.27053513506052534No Hit
ATCCTGAATGGCAAACTCCTTCTTTTCCCGACATTCATTTGCAGGAGGAC11730.26984499440986076No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11590.26662433804009256No Hit
ATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT11250.25880274399922704No Hit
TCCAGAGAGTATGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA11210.2578825564650076No Hit
GACTTAAATTAATAATGCCTGCCAGATTTTATCCTAAGGTTACCAAATAT10980.25259147814324556No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10920.2512111968419164No Hit
GAAAACAGGAGACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGGT10320.23740838382862428No Hit
GGATCATATTGTACAAAAAATCACACAATGTTTTAGGAAACTTCCTGTAA10290.23671824317795967No Hit
GTATCTGACCCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG10230.23533796187663047No Hit
GACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGGTTACCAAATAT9460.21762435184290557No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAAAGTCCAA9240.21256332040469847No Hit
GTGTTGACTCTCTTTTATATAAGATACCCGCCTTCCAGAGAGTATGCAAA9120.20980275780204002No Hit
GGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCAG8910.20497177324738783No Hit
GTATGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG8860.20382153882961349No Hit
GAAAAAAGGAGACTAAAATTAATAATGCCTGCCAGATTTTATCCTAAGGT8840.20336144506250373No Hit
ATATAAGATACCCGCCTTCCAGAGAGTATGCAAATAATGTCTCGTCTGGA8630.19853046050785148No Hit
TTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8000.18403750684389478No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7560.17391544396748057No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7390.1700046469470478No Hit
ATATAAAAGAGAGTCAACACATAGCGCCTCATTTTGCGGGTCACCATATT7340.16885441252927347No Hit
GTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAA7340.16885441252927347No Hit
TTATATGCCTGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTA7210.16586380304306017No Hit
CAGTCAATGAAAACAGGAGACTAAAATTAATAATGCCTGCCAGATTTTAT7070.16264314667329202No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACACGAG6810.15666192770086543No Hit
ATCCTAAGGTTACCAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT6800.15643188081731058No Hit
CCTGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6790.1562018339337557No Hit
ATCACACAATGTTTTAGGAAACTTCCTGTAAACAGGCCTATTGATTGGAA6720.15459150574887162No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA6490.14930042742710967No Hit
GTATATGACCCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG6460.14861028677644503No Hit
CTCCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGT6290.1446994897560123No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6180.14216897403690873No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAATA6080.13986850520136004No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6000.1380281301329211No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6000.1380281301329211No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTTTGTCAAAGAATTGTGGGTCT5720.1315868173933848No Hit
ACTCACATCTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5650.1299764892085007No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5650.1299764892085007No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5610.12905630167428123No Hit
GAGTATGCAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCG5600.12882625479072635No Hit
CATTTACTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCC5560.12790606725650688No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5440.12514550465384844No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5340.12284503581829977No Hit
GTCCCGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAGACGGGCAAC5280.12146475451697056No Hit
GGATATCCACATTGCGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT5160.11870419191431214No Hit
GCACGGGACCATGCAAGACCTGCACAACTCCTGCTCAAGGAACCTCTATG5110.1175539574965378No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA5050.11617367619520859No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4980.1145633480103245No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4940.11364316047610502No Hit
GATGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4860.1118027854076661No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT4820.1108825978734466No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCCTGTATACAAGCCAAACAG4820.1108825978734466No Hit
ATGTGTTGACTCTCTTTTATATAAGATACCCGCCTTCCAGAGAGTATGCA4780.10996241033922713No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCATCAACCACC4730.10881217592145279No Hit
TATCCACATTGCGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCT4730.10881217592145279No Hit
AGCTACCTGGGTGGGTAATAATTTACAAGATCCAGCGTCCAGGGATCTAG4670.10743189462012358No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4670.10743189462012358No Hit
CCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT4650.10697180085301385No Hit
GCGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCTTTGACAAACT4640.10674175396945898No Hit
CCCCAAAAGACCCACAATTCTTTGACAAACTTTCCAATCAATAGGCCTGT4620.10628166020234923No Hit
CTTTATCTAAGGGCAAATATTTGGTAACCTTAGGATAAAATCTGGCAGGC4570.10513142578457489No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4560.10490137890102001No Hit
GATTAACTACATGCTCGGGATAATAAGGTTTGATCCCTTTATCTAAGGGC4480.10306100383258107No Hit
CCCGATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4430.10191076941480674No Hit
ACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG4420.10168072253125186No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4400.10122062876414215No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATAATGCCTGCCAGA4390.10099058188058727No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCGATTACATATCCCATGAA4370.10053048811347752No Hit
CTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4360.10030044122992265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)