FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463913
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG35840.7725586478499201No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25810.5563543164343315No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT23850.5141050153800389No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16510.3558856940848823No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14930.32182758405132No Hit
TTATTAGTAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12310.26535147753996974No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12290.26492036222308923No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG11750.2532802486673148No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11620.25047799910759133No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10820.23323338643236988No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10450.22525775307007997No Hit
CTTTTATATAGAATGCCAGCCTTCCACATGGTATGTAAATAATGCCTAGT9020.19443300791312162No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8420.18149954840670557No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8340.17977508713918341No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8230.17740395289634048No Hit
TCCACATGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8140.17546393397037807No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7890.1700749925093714No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7750.16705718529120764No Hit
TTTATATAGAATGCCAGCCTTCCACATGGTATGTAAATAATGCCTAGTTT7630.1644704933899244No Hit
CTCCAAAAGACACCAAATACTCCAGAACAGTTTCTCTTCCAAAAGTAAGA7550.16274603212240227No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7520.16209935914708146No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7450.16059045553799958No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7320.1577882059782761No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7030.15153703388350834No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6950.14981257261598618No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6940.1495970149575459No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6840.14744143837314322No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6720.14485474647186003No Hit
CAATACTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCACATTT6670.1437769581796587No Hit
ATGTTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCACATTTCC6400.13795690140177144No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATA6350.13687911310957013No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6090.13127461399012316No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5840.12588567252911645No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5690.12265230765251244No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5660.12200563467719162No Hit
TTCTTCTACTGTACCTGTCTTCAATCCTGAGTGGCAAACTCCCTCCTTTC5430.11704780853306546No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5370.11575446258242385No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5360.11553890492398358No Hit
CTACTGTACCTGTCTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5300.11424555897334197No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5270.11359888599802118No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG5250.11316777068114064No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5070.1092877328292158No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5030.10842550219545474No Hit
CTCCAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5010.10799438687857423No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4980.10734771390325341No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4870.10497657966041045No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATATGTT4850.10454546434352993No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4750.10238988775912725No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4670.10066542649160512No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4640.10001875351628431No Hit
GTGTCGTTTCTCTTTTATATAGAATGCCAGCCTTCCACATGGTATGTAAA4640.10001875351628431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)