FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463913
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG37250.8029522776899979No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25710.5541987398499287No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT20440.4405998538519076No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG17440.37593255631982725No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14730.31751643088251463No Hit
TTATTAGTAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12380.26686038114905164No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT12050.25974697842052286No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11680.2517713450582329No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11180.2409934621362195No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG10520.22676665667916182No Hit
CTTTTATATAGAATGCCAGCCTTCCACATGGTATGTAAATAATGCCTAGT10260.22116215755971486No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10110.21792879268311086No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9300.20046862234944915No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8680.18710404752615253No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8340.17977508713918341No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8330.17955952948074316No Hit
TTTATATAGAATGCCAGCCTTCCACATGGTATGTAAATAATGCCTAGTTT8200.17675727992101967No Hit
CTCCAAAAGACACCAAATACTCCAGAACAGTTTCTCTTCCAAAAGTAAGA8160.1758950492872586No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8020.17287724206909486No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8000.17244612675221432No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7720.16641051231588683No Hit
TCCACATGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7350.15843487895359692No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7320.1577882059782761No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7090.15283037983414993No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7080.15261482217570968No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6870.14808811134846403No Hit
ATGTTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCACATTTCC6770.14593253476406137No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6650.14334584286277816No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATA6590.14205249691213653No Hit
CAATACTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCACATTT6110.1317057293070037No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5970.12868792208883992No Hit
GTGTCGTTTCTCTTTTATATAGAATGCCAGCCTTCCACATGGTATGTAAA5900.12717901847975804No Hit
CTACTGTACCTGTCTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5750.12394565360315404No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5750.12394565360315404No Hit
TTCTTCTACTGTACCTGTCTTCAATCCTGAGTGGCAAACTCCCTCCTTTC5740.12373009594471376No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5740.12373009594471376No Hit
CTCCAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5720.12329898062783323No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5670.12222119233563189No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5420.1168322508746252No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5400.11640113555774466No Hit
AGTCTGTACAACATCTTGAGGCCCTTTTTACCTCTATTACCAATTTTCTT5370.11575446258242385No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5160.11122775175517823No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATATGTT4950.10670104092793262No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4840.10432990668508967No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4800.10346767605132857No Hit
GTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4730.10195877244224671No Hit
TCTACTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT4720.10174321478380645No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAAT4660.10044986883316484No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4650.10023431117472457No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4650.10023431117472457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)