FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499624
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC22010.44053127952220067No Hit
CCTCAGGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18520.37067875042031606No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17220.344659183706147No Hit
AGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA15840.3170384128864906No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15350.3072310377403808No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG14440.2890173410404624No Hit
GTTCCGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA14410.28841688950090466No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA14370.28761628744816103No Hit
ACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTC14150.2832129761580709No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA12600.2521896466142539No Hit
CTCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC12380.24778633532416378No Hit
GTGCTGGTGTGGAGGGGGGATGAAGGGCCCAGGACATCTCTATGGGGTCT12080.2417818199285863No Hit
ATCCTGAACATGTAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT11790.23597745504619475No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11600.23217459529566234No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11540.23097369221654682No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC11090.22196691912318064No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA10740.2149616511616736No Hit
TTAATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGA10270.20555457704193553No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10090.20195186780458904No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA10070.20155156677821723No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG9790.19594735240901157No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9700.19414599779033834No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA9690.19394584727715242No Hit
ATCCTCTGGGATTCCTTCCCGATCACCAGTTGGACCCTGCGTTCGGAGCC9640.19294509471122284No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9470.18954253598706228No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA9400.18814148239476086No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9130.18273741853874112No Hit
GGATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA9100.1821369669991834No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8980.1797351608409524No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8700.17413094647174676No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8670.173530494932189No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8160.1633228187597073No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA8080.16172161465421997No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7920.1585192064432453No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA7840.156918002337758No Hit
GATTCGTCCCCATGCCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTG7750.15511664771908476No Hit
CATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAAT7720.15451619617952703No Hit
CCTCTTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7680.15371559412678334No Hit
ATACTACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7600.152114390021296No Hit
GTTATTAGATGTCGAGGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCG7600.152114390021296No Hit
ATTCTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA7540.1509134869421805No Hit
TAGGTATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGT7530.1507133364289946No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACTCACG7510.15031303540262278No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCCGG7430.14871183129713544No Hit
ATCCGGAACATGTAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT7330.1467103261652763No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG7310.14631002513890445No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGCGTTTG7280.14570957359934672No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG7250.14510912205978896No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7220.1445086705202312No Hit
AAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA7130.14270731590155797No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7050.14110611179607063No Hit
CCACATTAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGA7050.14110611179607063No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT6570.1314988871631467No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6520.1304981345972171No Hit
TCCCTACATAGAATGCTCAGGGGGCCAAGGGGGCGTCCAGGGACTTCATG6480.12969753254447344No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC6410.12829647895217203No Hit
GGGCACATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATC6400.12809632843898613No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6220.12449361920163964No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTGAAA6210.12429346868845371No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTGTGGTG6070.1214913615038509No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5950.11908955534561991No Hit
TCTCTATGGGGTCTACTTGGGACCAGACTGGCCATCAACACACAGCCCAG5940.118889404832434No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5860.11728820072694666No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5840.11688789970057482No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5820.116487598674203No Hit
ATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAGTTGATGAATTTG5730.11468624405552975No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG5650.11308503995004243No Hit
GCTAGAAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG5560.11128368533136919No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5540.11088338430499736No Hit
CTTTAATCCTGAGTGGCAAACGCCCTCCTTTCCTCACATTCATTTACAGG5540.11088338430499736No Hit
CTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5440.10888187917313819No Hit
CCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAAC5430.10868172865995229No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACTAATTTTCTT5400.10808127712039453No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA5400.10808127712039453No Hit
TAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGT5390.10788112660720862No Hit
GGTCTTATGAGGGTTCAAATGGATACCCAAAGACAAAAGAAAATTAGTAA5370.1074808255808368No Hit
CGAGAAGATTGACGATATGGGTGAGGCAGTAGTCGGAACAGGGTTTACTG5350.10708052455446494No Hit
GTTCTGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGTGAGG5300.10607977198853538No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5200.10407826685667622No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5190.10387811634349031No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTGGAGAGTCCAA5160.10327766480393255No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT5150.10307751429074666No Hit
GCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTGGGATAGAACCTAGCAG5130.1026772132643748No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT5120.1024770627511889No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5090.10187661121163114No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5080.10167646069844524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)