FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499624
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC19700.3942965109762542No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15440.30903239235905405No Hit
CCTCAGGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15390.3080316397931244No Hit
AGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA14280.2858149328294878No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14220.28461402975037225No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA13820.2766080092229356No Hit
GTTCCGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA12780.2557923558516004No Hit
ACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTC12290.24598498070549052No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11530.23077354170336095No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10470.20955758730565385No Hit
CTCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC10420.20855683473972428No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG10340.20695563063423697No Hit
ATCCTGAACATGTAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT10300.20615502858149332No Hit
GTGCTGGTGTGGAGGGGGGATGAAGGGCCCAGGACATCTCTATGGGGTCT10290.20595487806830737No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA9950.19914976061998624No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9870.19754855651449887No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA9560.1913438906057355No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9310.1863401277760876No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9140.18293756905192704No Hit
GGATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA9120.18253726802555523No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8900.17813395673546506No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG8830.17673290314316364No Hit
TTAATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGA8750.17513169903767634No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA8430.16872688261572702No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8220.1645237218388228No Hit
ATCCTCTGGGATTCCTTCCCGATCACCAGTTGGACCCTGCGTTCGGAGCC8210.16432357132563688No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACTCACG8020.16052071157510447No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7970.1595199590091749No Hit
ATTCTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA7960.15931980849598898No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7950.15911965798280306No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA7900.15811890541687348No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA7860.15731830336412983No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7840.156918002337758No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7770.15551694874545657No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA7590.1519142395081101No Hit
GATTCGTCCCCATGCCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTG7570.15151393848173825No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA7230.14470882103341715No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG7160.14330776744111573No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGCGTTTG7100.14210686436200023No Hit
ATACTACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6870.13750340255872415No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCCGG6860.13730325204553825No Hit
GTTATTAGATGTCGAGGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCG6760.13530174691367908No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6720.13450114486093542No Hit
ATCCGGAACATGTAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6600.13209933870270443No Hit
CATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAAT6480.12969753254447344No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT6450.12909708100491568No Hit
CCACATTAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGA6390.1278961779258002No Hit
CCTCTTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6150.12309256560933822No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6030.12069075945110724No Hit
TAGGTATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGT5940.118889404832434No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5900.11808880277969032No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTGTGGTG5860.11728820072694666No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5830.11668774918738893No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG5820.116487598674203No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5820.116487598674203No Hit
TCCCTACATAGAATGCTCAGGGGGCCAAGGGGGCGTCCAGGGACTTCATG5730.11468624405552975No Hit
GGGCACATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATC5660.11328519046322834No Hit
TCTCTATGGGGTCTACTTGGGACCAGACTGGCCATCAACACACAGCCCAG5650.11308503995004243No Hit
AAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA5510.1102829327654396No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5480.10968248122588187No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC5430.10868172865995229No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACTAATTTTCTT5410.10828142763358045No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTGAAA5400.10808127712039453No Hit
GCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTGGGATAGAACCTAGCAG5290.10587962147534946No Hit
CTTTAATCCTGAGTGGCAAACGCCCTCCTTTCCTCACATTCATTTACAGG5200.10407826685667622No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5160.10327766480393255No Hit
CCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAAC5130.1026772132643748No Hit
ATCTCCTCGAGGACTGGGGACCCTGCACCGAACATGGAGAGCACAACATC5050.10107600915888748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)