FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505828
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40830.8071913773061199No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14460.28586792348387197No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC13830.2734130969420435No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12270.24257257407656355No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT11890.2350601390195877No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11770.23268779110685844No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10100.1996726159880434No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9930.19631178977834365No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT9680.19136939829349106No Hit
GTATACATGCATATAAAGGCATTAGGGCAGGATAGCCACATTGTGTAAAA9590.18959013735894414No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9490.18761318076500313No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8790.17377448460741596No Hit
CCCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8680.17159983235408083No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8320.16448278861589316No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8060.1593427014716465No Hit
GGGATTTAGTAGTCGGCTATGTCAATGTTAATATGGGCTTAAAACTCAGG7880.15578417960255264No Hit
CAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGC7740.1530164403710352No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGAACAACAGAGT7660.1514348750958824No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACACCCTTAACTTCAT7610.1504463967989119No Hit
CCCTTAACTTCATGGGATATAGAATTGGAAGTTGGGGTACTTTACCACAG7270.1437247443795124No Hit
GTTCCTGTGGTAAAGTACCCCAACTTCCAATTCTATATCCCATGAAGTTA7210.1425385704231478No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6700.13245609179404857No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6440.12731600464980192No Hit
ATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG6420.1269206133310137No Hit
AGTTAAGGGTGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6400.1265252220122255No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6390.1263275263528314No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA6380.1261298306934373No Hit
ATTTACAGGCAGCTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT6310.12474596107767857No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6250.12355978712131396No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGCTTTCGAAAAC6210.12276900448373756No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6060.11980356959282602No Hit
GTCTATTTACAGGCAGCTTTCGAAAACATTGCTTGATTTTTAGTACAATA6050.1196058739334319No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTG6030.1192104826146437No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6020.1190127869552496No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5960.117826612998885No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5950.1176289173394909No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5950.1176289173394909No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCTTAAAACTCAGG5890.11644274338312628No Hit
GTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAG5720.11308191717342654No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC5640.11150035189827373No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA5620.11110496057948553No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5610.11090726492009141No Hit
TCTATATCCCATGAAGTTAAGGGTGTAGCCCCAACGTTTGGTTTTATTAG5310.10497639513826834No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5300.10477869947887425No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5300.10477869947887425No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGAAAGCTGCCTGTAA5290.10458100381948014No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5250.10379022118190374No Hit
CTGGCTGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTG5220.10319713420372144No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTCTATATCCCATGAA5140.1016155689285686No Hit
CTGTAAGGGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5060.10003400365341578No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5060.10003400365341578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)