FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505828
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40510.8008651162055087No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14870.2939734455190302No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11100.2194421819274536No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11100.2194421819274536No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11090.21924448626805948No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11080.2190467906086654No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10330.20421961615410775No Hit
GTATACATGCATATAAAGGCATTAGGGCAGGATAGCCACATTGTGTAAAA10210.2018472682413785No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT10140.2004633986256198No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9160.18108922400499775No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8900.1759491368607511No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8630.17061135405711034No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8070.15954039713104057No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGAACAACAGAGT7990.15795883185588777No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT7820.15459800564618803No Hit
GTTCCTGTGGTAAAGTACCCCAACTTCCAATTCTATATCCCATGAAGTTA7760.15341183168982342No Hit
CCCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7740.1530164403710352No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7570.14965561416133547No Hit
GGGATTTAGTAGTCGGCTATGTCAATGTTAATATGGGCTTAAAACTCAGG7530.14886483152375907No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACACCCTTAACTTCAT7050.13937543987284215No Hit
GTCTATTTACAGGCAGCTTTCGAAAACATTGCTTGATTTTTAGTACAATA6830.1350261353661719No Hit
CAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGC6820.1348284397067778No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6610.13067683085950166No Hit
ATTTACAGGCAGCTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT6560.12968835256253114No Hit
CCCTTAACTTCATGGGATATAGAATTGGAAGTTGGGGTACTTTACCACAG6440.12731600464980192No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6410.1267229176716196No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6330.1251413523964668No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGCTTTCGAAAAC6320.12494365673707268No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA6170.12197822184616114No Hit
AGTTAAGGGTGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6110.12079204788979653No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6100.12059435223040244No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTG6070.12000126525222013No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA5950.1176289173394909No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5940.11743122168009679No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5890.11644274338312628No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5810.11486117810797347No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5750.11367500415160885No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5710.11288422151403243No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5550.1097210909637268No Hit
ATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG5510.10893030832615039No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5480.10833722134796807No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5460.10794183002917987No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5440.10754643871039168No Hit
CTGTAAGGGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5280.10438330816008602No Hit
CTGGCTGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTG5250.10379022118190374No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5210.10299943854432732No Hit
GTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAG5170.1022086559067509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)