FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551711
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG26930.48811787330685813No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAACCTTAT24930.4518670100831776No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16380.2968945698019434No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15470.28040042703516876No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAATACAAT15210.27568781481609034No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTAAGA14020.2541185511980004No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT13670.2477746501338563No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12830.23254928757991047No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA12590.22819918399306885No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA12480.22620538651576644No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA12390.22457409767070077No Hit
GGATAATAAGGTTTAATTCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT12350.22384908040622717No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC12350.22384908040622717No Hit
CCCTTAACTTTATGGGTTATGTAATTGGAAGTTGGGGTACTTTACCACAG11970.2169614163937279No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAAC11920.21605514481313587No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT11850.21478636460030703No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGAATGTTCAACATCTTG10990.19919849341412443No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10500.1903170319243227No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGAATGTTCAACATCTTGAG10270.18614818265359945No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10260.18596692833748105No Hit
CATTAACATAGCTGACTACTGCTTCCCTGGATTGTGGATCTTCCAAATTA9970.18071055317004736No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT9920.17980428158945536No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT9080.16457891903550953No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAACCCATAAA8930.1618601042937335No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8870.16077257839702308No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATTCC8810.15968505250031267No Hit
ATGTTAATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCAC8730.15823501797136544No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTTTGACATACT8670.15714749207465503No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8580.1555162032295894No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA8530.1546099316489974No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG8370.15170986259110295No Hit
ATATAAAAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATATT8190.1484472849009717No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTTAT7980.14464094426248525No Hit
CTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCT7950.14409718131413005No Hit
ATCCAGATTGGGACTTCAACCCCAACAAGGATCACTGGCCAGAGGCAACT7880.14282840110130124No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTGTTGG7850.142284638152946No Hit
AATCCGGATTGTTTGAATTGGCTCCGAACGCAGGGTCCAACTGGTGATCG7620.13811578888222276No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7510.13612199140492034No Hit
GTTCAGGATAATAAGGTTTAATTCCTTTGTCCAAGGGCAAATATTTGGTA7420.1344907025598547No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA7410.1343094482437363No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7400.13412819392761788No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC7360.13340317666314427No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7350.1332219223470259No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7270.13177188781807866No Hit
ATGTATCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG7240.13122812486972346No Hit
GTATCGGGATGCCTTAGAGTCTCATGAACATTGTTCAGCTCACCATACAG7170.12995934465689465No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7130.12923432739242105No Hit
CTTTATGGGTTATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA6900.12506547812169777No Hit
GTTCTTGAGTATTTGGTATCTTTTGGAGTGTGGATTCGCACTCCTACCGC6880.12470296948946097No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATAACCCATAAAGTTAAGGGAG6780.12289042632827693No Hit
GGTCGATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAATACAAT6710.12162164611544811No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6710.12162164611544811No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6700.12144039179932972No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6630.1201716115865009No Hit
CCCATAAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAA6620.11999035727038251No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATTCCTTTGTCCAAGGGC6580.11926534000590888No Hit
AGTCTAGATTCTGTAGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6580.11926534000590888No Hit
GTCCAAGGGATACTAACATTGAGAGTCCCGAGATTGAGATCTTCTGCGAC6520.11817781410919848No Hit
CTGTAGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA6480.11745279684472487No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6470.11727154252860646No Hit
ATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG6380.11564025368354083No Hit
ATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATCTTGTGGTTTCAC6360.11527774505130403No Hit
ATACTACAGAATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6360.11527774505130403No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6360.11527774505130403No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6330.11473398210294884No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA6300.11419021915459363No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6300.11419021915459363No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6250.1132839475740016No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGG6210.112558930309528No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAACCC6050.10965886125163356No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5990.10857133535492314No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTGTTGG5960.10802757240656793No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5960.10802757240656793No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAATACAATA5940.10766506377433112No Hit
GGAACATATTGTATTAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAA5910.10712130082597592No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5900.10694004650985751No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5890.1067587921937391No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATTCCTTTGTCCAAGG5880.10657753787762071No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5880.10657753787762071No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5840.10585252061314711No Hit
CATTGACATAGCTGACTACTGCTTCCCTGGATTGTGGATCTTCCAAATTA5710.10349621450360785No Hit
GAATATGGTGACCCACAAAATGAAGCGCTTCGTGTAGTTTCTCTTTTATA5680.10295245155525266No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5660.10258994292301586No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5660.10258994292301586No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5660.10258994292301586No Hit
ATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTT5630.10204617997466064No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC5600.10150241702630543No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG5590.10132116271018704No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5580.10113990839406863No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5580.10113990839406863No Hit
GTTCCGTTTCTTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTC5540.10041489112959502No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT5540.10041489112959502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)