FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551711
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG27140.4919242139453446No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAACCTTAT21160.38353413290653987No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15380.2787691381901031No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAATACAAT14990.27170021986148546No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14380.2606437065782629No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTAAGA14120.25593109435918443No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT14040.2544810598302372No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12780.2316430159993185No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA12380.2243928433545824No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA12380.2243928433545824No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT12170.22058650271609592No Hit
CCCTTAACTTTATGGGTTATGTAATTGGAAGTTGGGGTACTTTACCACAG11990.21732392502596468No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGAATTAAAC11830.21442385596807026No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA11450.20753619195557094No Hit
GGATAATAAGGTTTAATTCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT11390.20644866605886053No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC10890.1973859502529404No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGAATGTTCAACATCTTG10790.19557340709175636No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT10720.19430462687892755No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCTTTGACATACT10180.18451689380853384No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA9830.17817299274438972No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9550.17309787189307446No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG9540.17291661757695603No Hit
CATTAACATAGCTGACTACTGCTTCCCTGGATTGTGGATCTTCCAAATTA9410.17056031146741682No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGAATGTTCAACATCTTGAG9330.1691102769384696No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT9190.16657271651281197No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8810.15968505250031267No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAACCCATAAA8710.15787250933912864No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA8620.15624122049406303No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC8560.1551536945973526No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATTCC8530.1546099316489974No Hit
ATATAAAAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATATT8340.15116609964274774No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTGTTGG8100.1468159960559061No Hit
ATGTTAATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCAC7980.14464094426248525No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7860.14246589246906444No Hit
GTTCTTGAGTATTTGGTATCTTTTGGAGTGTGGATTCGCACTCCTACCGC7850.142284638152946No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTTAT7800.141378366572354No Hit
ATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7760.1406533493078804No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7590.13757202593386755No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7570.13720951730163075No Hit
GTTCAGGATAATAAGGTTTAATTCCTTTGTCCAAGGGCAAATATTTGGTA7540.13666575435327552No Hit
GTATCGGGATGCCTTAGAGTCTCATGAACATTGTTCAGCTCACCATACAG7440.1348532111920915No Hit
AATCCGGATTGTTTGAATTGGCTCCGAACGCAGGGTCCAACTGGTGATCG7310.1324969050825523No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7220.13086561623748666No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA7200.13050310760524983No Hit
ATCCAGATTGGGACTTCAACCCCAACAAGGATCACTGGCCAGAGGCAACT7180.13014059897301303No Hit
CTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCT7170.12995934465689465No Hit
ATGTATCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG7010.1270592755990002No Hit
ATACTACAGAATCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6970.1263342583345266No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6860.12434046085722417No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6850.12415920654110575No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6780.12289042632827693No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6770.12270917201215854No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA6760.12252791769604013No Hit
CCCATAAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAA6550.11872157705755369No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6530.11835906842531689No Hit
CTTTATGGGTTATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA6520.11817781410919848No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6510.11799655979308006No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGG6490.11763405116084326No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6470.11727154252860646No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6440.11672777958025127No Hit
GGTCGATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAATACAAT6410.11618401663189605No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6350.11509649073518564No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC6160.11165265872893598No Hit
AGTCTAGATTCTGTAGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6160.11165265872893598No Hit
ATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACCTG6130.11110889578058078No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6130.11110889578058078No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6080.11020262419998876No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6010.10893384398715994No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTGTTGG5960.10802757240656793No Hit
GTCCAAGGGATACTAACATTGAGAGTCCCGAGATTGAGATCTTCTGCGAC5880.10657753787762071No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5870.1063962835615023No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5860.10621502924538391No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAAGTTTTAATACAATA5830.1056712662970287No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATTCCTTTGTCCAAGGGC5820.10549001198091029No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5790.10494624903255509No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATAACCCATAAAGTTAAGGGAG5770.10458374040031829No Hit
TTTCTGTGCCCAATCCCCTGGGATTCTTTCCCGATCACCAGTTGGACCCT5740.10403997745196307No Hit
CATTGACATAGCTGACTACTGCTTCCCTGGATTGTGGATCTTCCAAATTA5720.10367746881972627No Hit
GTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5720.10367746881972627No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAACCC5670.10277119723913425No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5640.10222743429077906No Hit
CTGTAGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5630.10204617997466064No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT5580.10113990839406863No Hit
ATATAGACCCCTCACTCTGGGATCTAGCAGAGCTTGTTTGAATGTTGTGG5560.10077739976183182No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5550.10059614544571344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)