FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477658
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19730.41305704081162675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14370.3008428624664509No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCCTAT13330.27906996219052127No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC12820.2683928668629019No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10890.2279873884662248No Hit
CTTATATAGAATTCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9840.20600513337994966No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACTTTACCGCAA8880.18590707158678385No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8750.18318545905229267No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8170.17104288005225496No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8070.16894933194880019No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8010.16769320308672733No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACATAAGA7990.16727449346603637No Hit
GCCCAACAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6720.14068643255216076No Hit
GGATAATAGGGTTTAATGCCCTTGTCCAAGGGCAAATATTTGGTAAGGTT6640.13901159406939692No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6630.13880223925905147No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6520.13649933634525122No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6420.13440578824179644No Hit
CTCTTATATAGAATTCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT6300.1318935305176507No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAAC6300.1318935305176507No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6110.12791578912108664No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6070.12707836987970472No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG6040.12645030544866828No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6030.12624095063832283No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT5940.12435675734521352No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5930.12414740253486804No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTAGTAAT5670.11870417746588564No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5400.11305159758655775No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5330.1115861139141394No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5320.11137675910379392No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5290.1107486946727575No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC5230.10949256581068464No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5090.10656159846584795No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5080.10635224365550247No Hit
GTCAACATTAATATGGGCCTAAAATTCAGACAAATACTGTGGTTTCACGT5000.10467740517273866No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4960.10383998593135675No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4900.10258385706928387No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA4870.10195579263824746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)