FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477658
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG22040.461418002001432No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14270.2987493143629961No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11910.24934157912146349No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCCTAT11600.2428515800007537No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9750.20412094008684037No Hit
CTTATATAGAATTCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8850.1852790071557474No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8810.18444158791436552No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACTTTACCGCAA8730.1827667494316017No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACATAAGA8700.18213868500056526No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8530.17857965322469216No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8230.1722990089143278No Hit
GCCCAACAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7750.16224997801774493No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6960.1457109480004522No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6900.14445481913837935No Hit
GGATAATAGGGTTTAATGCCCTTGTCCAAGGGCAAATATTTGGTAAGGTT6590.13796482001766955No Hit
CTCTTATATAGAATTCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT6360.13314965937972356No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6230.13042804684523238No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6130.12833449874177758No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAAC6000.1256128862072864No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5910.12372869291417708No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT5820.12184449962106779No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG5810.12163514481072232No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5810.12163514481072232No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTAGTAAT5700.11933224189692206No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5330.1115861139141394No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5270.11032998505206655No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC5240.10970192062103011No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5230.10949256581068464No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5130.10739901770722986No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5120.10718966289688438No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5100.10677095327619342No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5000.10467740517273866No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4980.10425869555204771No Hit
GTCAACATTAATATGGGCCTAAAATTCAGACAAATACTGTGGTTTCACGT4930.10321192150032031No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4910.10279321187962936No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4910.10279321187962936No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4870.10195579263824746No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4870.10195579263824746No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4850.1015370830175565No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4840.10132772820721102No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG4830.10111837339686552No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4820.10090901858652007No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4790.10028095415548363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)