FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345058
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA14130.40949637452254406No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT10630.308064151533945No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10430.3022680245060251No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10430.3022680245060251No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9750.28256119261109724No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9450.2738670020692174No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9200.26662184328431743No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8030.23271450017098577No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7740.22431011598050182No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7720.22373050327770985No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7410.21474650638443393No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6940.20112560786882203No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6780.19648870624648607No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6350.1840270331364582No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6200.17967993786551828No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA6150.17823090610853828No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6000.17388381083759832No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5840.16924690921526236No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCAC5780.16750807110688637No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG5690.1648998139443224No Hit
CCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5490.15910368691640248No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5410.15678523610523448No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5400.15649542975383848No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5380.15591581705104648No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5280.15301775353708652No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT5160.14954007732033456No Hit
GTCTATTTACAGGCAGTTTTCGAAACAATTGCTTGATTTTAAGTACAATA5150.14925027096893856No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5130.14867065826614656No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5110.14809104556335456No Hit
GCTTTAGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCTTCTGTGG4940.1431643375896226No Hit
CTTATATAAAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT4890.14171530583264264No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4750.13765801691309867No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4740.13736821056170267No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4740.13736821056170267No Hit
GTCCCGTGGTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4730.13707840421030668No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTGTTGTC4680.13562937245332668No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4670.13533956610193068No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4660.13504975975053468No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4650.1347599533991387No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4590.13302111529076271No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4550.13186188988517872No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4540.13157208353378272No Hit
ATATCGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCA4500.13041285812819872No Hit
TGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTG4430.12838421366842676No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACC4390.12722498826284276No Hit
AGACTGTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTG4380.1269351819114468No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATT4310.12490653745167478No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4290.1243269247488828No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4290.1243269247488828No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4280.1240371183974868No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC4260.12345750569469481No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4230.12258808664050681No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG4100.11882060407235887No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4070.11795118501817085No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4060.11766137866677487No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4050.11737157231537887No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4040.11708176596398287No Hit
CTCTTATATAAAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGT3830.11099583258466691No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3820.11070602623327094No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3790.10983660717908293No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTTTATATAAGAGAGAAACTACA3780.10954680082768695No Hit
ACATTGACCTGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3770.10925699447629095No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG3770.10925699447629095No Hit
CTGCCTGTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATAT3750.10867738177349894No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3730.10809776907070695No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTG3730.10809776907070695No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3730.10809776907070695No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT3710.10751815636791495No Hit
ATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGAATCTG3640.10548951190814297No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3610.10462009285395499No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3580.103750673799767No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3490.10114241663720303No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3490.10114241663720303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)