FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345058
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA10580.30661511977696504No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10400.3013986054518371No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT10010.2900961577473932No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9970.28893693234180917No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9900.2869082878820372No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9780.28343061166528527No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9450.2738670020692174No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8500.24633539868659762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8050.23329411287377771No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8050.23329411287377771No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8020.23242469381958974No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6530.18924354746158617No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG6400.1854760648934382No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6300.18257800137947824No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCAC6140.17794109975714228No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6120.1773614870543503No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5990.17359400448620232No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5890.17069594097224233No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5790.16779787745828237No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5720.1657692329985104No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5500.15939349326779845No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5430.15736484880802648No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACC5420.15707504245663048No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5420.15707504245663048No Hit
CCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5420.15707504245663048No Hit
CTGCCTGTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATAT5410.15678523610523448No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT5220.15127891542871053No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5180.15011969002312653No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5140.14896046461754256No Hit
GTCTATTTACAGGCAGTTTTCGAAACAATTGCTTGATTTTAAGTACAATA5130.14867065826614656No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5110.14809104556335456No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5080.1472216265091666No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4980.1443235629952066No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4840.14026627407566264No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4830.13997646772426664No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4790.13881724231868264No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4680.13562937245332668No Hit
ATATCGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCA4680.13562937245332668No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4590.13302111529076271No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG4480.12983324542540675No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4480.12983324542540675No Hit
GTCCCGTGGTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4460.12925363272261473No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4430.12838421366842676No Hit
CTTATATAAAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT4400.12751479461423876No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATT4380.1269351819114468No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4310.12490653745167478No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4290.1243269247488828No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4230.12258808664050681No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4180.12113905488352683No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4140.11997982947794285No Hit
GCTTTAGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCTTCTGTGG4130.11969002312654685No Hit
CTCTTATATAAAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGT4030.11679195961258686No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4020.11650215326119089No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4000.11592254055839887No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3950.11447350880141888No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3840.11128563893606291No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3810.11041621988187493No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3780.10954680082768695No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC3780.10954680082768695No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCCTGTTCAGG3740.10838757542210296No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTGTTGTC3740.10838757542210296No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC3730.10809776907070695No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3600.10433028650255899No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT3560.10317106109697499No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3550.10288125474557899No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3550.10288125474557899No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3530.10230164204278701No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTG3530.10230164204278701No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3530.10230164204278701No Hit
TGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTG3520.10201183569139101No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3520.10201183569139101No Hit
ACATTGACCTGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3510.10172202933999501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)