FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424926
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23030.5419767206525371No Hit
GTTAATATCCCTTGGACGCATAAGGTGGGAAACTTTACTGGGCTTTATTC10390.24451316229178727No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAAGGAGTTTG9800.2306283917670371No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTGACTGTTAATGAAAAA9220.21697895633592673No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC8230.1936807820655832No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7730.1819140273835915No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7630.17956067644719317No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6820.16049853386236662No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAAGGAGTTTG6770.15932185839416746No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5930.13955371052842142No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5920.1393183754347816No Hit
CTTATATAGAATGCCAGCCTGCCACAGAGTATGTAAATAATGCCTAGTTT5800.13649435431110357No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5590.13155231734466707No Hit
GTACACCATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5580.13131698225102725No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5460.12849296112734923No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA5360.1261396101909509No Hit
GTTCCGGATAATAAGGTTTAATGCCTTTCTCTAAGGGGAAATATTTGGTA5310.12496293472275173No Hit
CTACTGTACCTGTCTTTAATTCTAAATGGCAAACTCCTTCCTTTCCTAAC5300.1247275996291119No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5230.12308025397363305No Hit
GCTCTATACCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA4840.11390218532167955No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT4760.11201950457256087No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG4660.10966615363616253No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4610.10848947816796337No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4600.10825414307432353No Hit
CTACTGTACCTGTCTTTAATCCTAAATGGCAAACTCCTTCCTTTCCTAAC4470.10519478685700569No Hit
ATATGTTCCTGAGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4460.10495945176336587No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCTCAG4430.10425344648244636No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4360.10260610082696753No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4300.10119409026512852No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4260.10025274989056918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)