FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424926
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22840.5375053538733803No Hit
GTTAATATCCCTTGGACGCATAAGGTGGGAAACTTTACTGGGCTTTATTC10810.25439723622466026No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAAGGAGTTTG9880.23251107251615577No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTGACTGTTAATGAAAAA9840.23156973214159643No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8850.20827155787125287No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC8570.20168217524933751No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7910.1861500590691085No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7210.16967660251432015No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAAGGAGTTTG6310.1484964440867351No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG5870.13814169996658243No Hit
GTTCCGGATAATAAGGTTTAATGCCTTTCTCTAAGGGGAAATATTTGGTA5830.1372003595920231No Hit
GTACACCATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5730.13484700865562474No Hit
CTTATATAGAATGCCAGCCTGCCACAGAGTATGTAAATAATGCCTAGTTT5730.13484700865562474No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5620.1322583226255866No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA5540.1303756418764679No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5330.1254336049100314No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5230.12308025397363305No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4910.11554953097715837No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4890.11507886078987871No Hit
GCTCTATACCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA4820.11343151513439986No Hit
CCTTTACCCCGTTGCCCGGCAACGAGCTGGTCTTTGCCAAGTGTTTGCTG4770.1122548396662007No Hit
CTACTGTACCTGTCTTTAATTCTAAATGGCAAACTCCTTCCTTTCCTAAC4670.10990148872980236No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4650.1094308185425227No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT4450.10472411666972602No Hit
ATATGTTCCTGAGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4410.10378277629516669No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4370.10284143592060736No Hit
GGATATTAACATTGGGATTGCCGAGATTGAGATCTTCTGCGACGCGGCGA4310.10142942535876834No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4250.10001741479692934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)