FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307045
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10340.33675845560097056No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10170.33122180787832406No Hit
GTATGTAAATAATGCCTAACTTTGAAGTAATGATTAACTGCATGTTCCGG10090.3286163265970786No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGATC8090.2634792945659431No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7970.259571072644075No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6710.2185347424644596No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6560.21364946506212446No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6400.20843850249963358No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6040.1967138367340292No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT5540.18042957872624535No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5430.17684704196453288No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5340.17391587552313179No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA5290.17228744972235338No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5100.16609943167939553No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5050.16447100587861715No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4910.15991141363643765No Hit
CCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4690.15274634011301275No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4200.13678776726538455No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4110.13385660082398346No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4100.13353091566382777No Hit
CCTTTATACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGA3970.12929700858180398No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3910.1273428976208699No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3790.12343467569900177No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3770.12278330537869042No Hit
GTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTTCTGTC3760.12245762021853475No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3720.12115487957791203No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCACCTTACAGACCACCAAATGCC3700.12050350925760067No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA3620.11789802797635526No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3570.11626960217557689No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3540.11529254669510984No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3520.11464117637479847No Hit
GAGTATGTAAATAATGCCTAACTTTGAAGTAATGATTAACTGCATGTTCC3510.1143154912146428No Hit
TCCACAGAGTATGTAAATAATGCCTAACTTTGAAGTAATGATTAACTGCA3490.11366412089433145No Hit
ATGTTAATATGGGCCTAAAAATCAGACAAATATTGTGGTTTCACATTTCC3470.11301275057402009No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG3440.11203569509355307No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC3400.11073295445293035No Hit
CTGATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAACTT3360.10943021381230765No Hit
GTTGTCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3310.10780178801152926No Hit
CTTTATACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA3300.10747610285137357No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGGCTGTCTCTTAGAGGTGG3220.10487062157012816No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3220.10487062157012816No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCGTCCTTTCCTAAC3210.10454493640997248No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3180.10356788092950545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)