FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307045
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAACTTTGAAGTAATGATTAACTGCATGTTCCGG11120.3621618980931134No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10330.3364327704408149No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9210.299956032503379No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGATC8600.28008923773388267No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7940.25859401716360797No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7380.24035564819489No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6540.2129980947418131No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6150.20029637349574164No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5600.18238368968717938No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5400.17586998648406585No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5380.1752186161637545No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5060.1647966910387728No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4930.160562783956749No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA4910.15991141363643765No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4700.15307202527316843No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4660.15176928463254571No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT4500.1465583220700549No Hit
CCTTTATACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGA4380.14265010014818674No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4160.13548502662476183No Hit
CCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4090.13320523050367208No Hit
GTACAACACCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTTCTGTC4040.1315768047028937No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4010.13059974922242668No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCACCTTACAGACCACCAAATGCC3880.1263658421404029No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3870.12604015698024718No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3760.12245762021853475No Hit
ATGTTAATATGGGCCTAAAAATCAGACAAATATTGTGGTTTCACATTTCC3630.11822371313651094No Hit
CTTTATACCTCTATTACCAATTTTCTTCTGTCTTTGGGTATACATTTGAA3610.11757234281619958No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3600.1172466576560439No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3580.11659528733573256No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA3560.11594391701542119No Hit
CTGATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAACTT3490.11366412089433145No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3460.11268706541386442No Hit
TCCACAGAGTATGTAAATAATGCCTAACTTTGAAGTAATGATTAACTGCA3430.11171000993339737No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3360.10943021381230765No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATCAGAGAGAAACTACA3250.10584767705059518No Hit
GAGTATGTAAATAATGCCTAACTTTGAAGTAATGATTAACTGCATGTTCC3230.10519630673028384No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC3210.10454493640997248No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3210.10454493640997248No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3130.10193945512872707No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAATA3090.10063671448810435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)