FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450946
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG31560.6998620677420356No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18440.4089181409747508No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15350.34039552407605345No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14130.3133412869833639No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA13660.30291875302142607No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC12780.28340422134801063No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11890.26366793363285185No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA10270.22774345487042796No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10220.22663467466171117No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10080.22353009007730415No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9870.21887321320069367No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9570.2122205319483929No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8790.19492356069241107No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8570.1900449277740572No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8180.18139644214606626No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7610.1687563477666949No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA7590.1683128356832082No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7500.166317031307518No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7470.16565176318228791No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT7360.16321244672311097No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7280.16143839838916413No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7230.16032961818044733No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7220.16010786213870395No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7170.15899908192998718No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6880.1525681567194298No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6800.15079410838548296No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6780.15035059630199624No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA6710.14879830400979274No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6610.14658074359235918No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG6610.14658074359235918No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTACGCGGGACGTCC6570.14569371942538575No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6400.14192386671574866No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6190.13726698983913815No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6140.13615820963042138No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6100.13527118546344793No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6040.13394064921298782No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTCCCTAAC5980.13261011296252764No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTCC5980.13261011296252764No Hit
ATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA5820.12906201629463396No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5760.12773148004417378No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5660.12551391962674022No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5500.12196582295884652No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5420.12019177462489965No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5400.11974826254141295No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA5300.11753070212397938No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5300.11753070212397938No Hit
CTATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATA5230.11597840983177587No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5210.11553489774828915No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5120.11353909337259893No Hit
GGAACATATTGTGCAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA5110.11331733733085558No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5100.11309558128911222No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5100.11309558128911222No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5090.11287382524736887No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5000.11087802087167864No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT5000.11087802087167864No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4980.11043450878819193No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4950.10976924066296186No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4940.10954748462121851No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTCCCTAACATTCATTTACAGG4940.10954748462121851No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4900.10866046045424507No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4870.107995192329015No Hit
ATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGGAT4840.10732992420378494No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4840.10732992420378494No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4830.10710816816204156No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4830.10710816816204156No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4810.10666465607855485No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4790.10622114399506816No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4730.104890607744608No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4680.10378182753589121No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4670.10356007149414787No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4620.10245129128543108No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4620.10245129128543108No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4590.101786023160201No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4560.10112075503497092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)