FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609559
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22560.37010363229810406No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21070.3456597310514651No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC16520.2710156030835407No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT10480.1719275738689774No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10330.1694667784414634No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAGGGCATTAAACCTTAT9040.1483039377648431No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8990.14748367262233844No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT8760.143710452966817No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8570.14059344542529928No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8510.13960912725429367No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8440.13846075605478714No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT8400.13780454394078342No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8390.13764049091228248No Hit
ATTGACAACAGTGCCAGCGGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8040.13189863491474985No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCACAG8000.1312424228007461No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7860.12894568040173307No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7460.12238355926169574No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA7300.11975871080568082No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7230.1186103396061743No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6760.11089984726663046No Hit
CATTTGAACCCTCATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6710.1100795821241258No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6510.10679852155410716No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAGGATTAATTATGCCTGCTAGG6200.10171287767057824No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6160.1010566655565745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)