FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435682
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG25020.574272060814998No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17140.39340619993481485No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13760.31582668092783267No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12160.27910264826180564No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11980.2749711945868776No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11960.27451214417855224No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11150.25592060264137606No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT11150.25592060264137606No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10700.24559196845405593No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTTTGGGTCACCATA10400.23870621232917588No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10100.2318204562042958No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT9600.2203441959961623No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8570.19670309996740742No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8490.19486689833410606No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT8460.19417832272161806No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA8450.1939487975174554No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA8420.1932602219049674No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTTTGGGTCACCATATT8280.19004686904669002No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA8170.18752209180090065No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGACA8110.18614494057592462No Hit
GGATCATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA7690.17650488200109254No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7470.1714553275095138No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7300.16755339903874844No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7210.16548767220128444No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7000.16066764291386837No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6960.1597495420972177No Hit
CATTCTTGATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA6790.1558476136264523No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA6650.15263426076817496No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6600.1514866347473616No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6560.15056853393071093No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6490.14896185750157223No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6460.14827328188908423No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATCAATAATGTCCTCCTGT6420.14735518107243356No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6410.1471256558682709No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC6380.1464370802557829No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6310.1448304038266442No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6270.14391230300999353No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC6120.1404694249475535No Hit
ATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6120.1404694249475535No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA6090.1397808493350655No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA5940.13633797127262542No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5800.13312461841434808No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA5790.1328950932101854No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC5700.13082936637272138No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5560.12761601351444402No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5550.12738648831028135No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5520.12669791269779335No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5500.126238862289468No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5480.12577981188114268No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5450.12509123626865468No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5400.12394361024784131No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5370.12325503463535331No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTG5370.12325503463535331No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5350.12279598422702796No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT5260.12073025738956394No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC5260.12073025738956394No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5230.12004168177707594No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5150.11820548014377459No Hit
CTTTACCACAGGATCATATTGTACTAAAAATCAAGCAATGTTTTCGGAAA5080.1165988037146359No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5050.1159102281021479No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG5050.1159102281021479No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5040.11568070289798522No Hit
GTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAACATCTTGAG5030.11545117769382256No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4970.11407402646884655No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4960.11384450126468387No Hit
ATTGATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG4950.1136149760605212No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTCGGAAGAGAAACTGTTCT4900.11246735003970787No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4880.11200829963138252No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4830.11086067361056919No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG4770.10948352238559315No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGGAGATTGACGATATGG4730.10856542156894247No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4700.10787684595645447No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4690.1076473207522918No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4680.10741779554812915No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4660.1069587451398038No Hit
GTTTTCCACTCAGGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGT4660.1069587451398038No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4610.10581111911899047No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4600.10558159391482778No Hit
CAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG4590.10535206871066512No Hit
GTCCAAGGGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGAC4580.10512254350650244No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4530.10397491748568911No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATATGAT4530.10397491748568911No Hit
ATCAATAATGTCCTCCTGTAAATGGATGTTAGGAAAGGAGGGAGTTTGCC4520.10374539228152643No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4510.10351586707736377No Hit
GTACAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4470.10259776626071308No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACAAAATGCC4450.10213871585238775No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4450.10213871585238775No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4420.10145014023989975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)