FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711591
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA48750.685084549973229No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36870.5181347150259068No Hit
CTCTATGGAAGGCGGGTATATTATATAAAAGAGAAACAACACATAGCGCC18780.26391564817430235No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT16160.22709674518086934No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16050.22555091337580158No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG15670.22021076714011278No Hit
CCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGG14890.20924941434054112No Hit
GGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG13910.19547745825902801No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAAC13690.1923857946488924No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT13630.19154261366431No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT13450.18901307071056267No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT13440.1888725405464656No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12530.176084295613632No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGAGTTTTTGT12450.17496005430085543No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC12450.17496005430085543No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA12110.17018202872155494No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG11880.16694983494732227No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11770.16540400314225448No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC11420.16048544739885692No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11140.1565506028041389No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC10850.1524752280453238No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACATT10630.1493835644351882No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT10340.1453081896763731No Hit
CACTAATATGGGCCTAAAGTTCAGGCAGCTATTGTGGTTTCACATTTCTT10060.14137334508165506No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTGTTTTC9900.1391248624561019No Hit
CTTTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9680.1360331988459663No Hit
CTCTTTACATTTTATGGGTTATGTCATTGGATGTTATGGGTCATTGCCAC9640.135471078189578No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG9550.13420630671270434No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9390.13195782408715118No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9230.12970934146159802No Hit
ATCCAAATGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT9190.12914722080520974No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG9030.12689873817965658No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA8830.12408813489771511No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTGTTTTCCT8820.12394760473361806No Hit
GTGTAAATGTATATTAGGAAAACAGGGTGTTTTCCAATGAGGGTTAAAGA8540.12001276013890001No Hit
ATATTAGTGTTGACATAACTGACTACTAGATCCCTGGATGTTGGATCTTC8460.11888851882612343No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC8170.11481314406730833No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8140.11439155357501711No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8090.11368890275453175No Hit
CTACACACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7970.11200254078536688No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT7950.11172148045717273No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7930.11144042012897859No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7900.11101882963668738No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7860.11045670898029906No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCACCAGC7570.10638133422148396No Hit
GGGTAAAGGTTCAGGTATTGCTTACACAGAAAGGCCTTGTAAGTTGGCGA7480.10511656274461033No Hit
CATCTTTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAACAGGGTGT7340.1031491404472513No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGAGTTTTTGTCTGATGTGAT7210.10132224831398935No Hit
GTTATGGGTCATTGCCACAAGATCACATCAGACAAAAACTCAAAGAATGT7150.10047906732940691No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTA7140.10033853716530985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)