FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388973
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16180.41596717509955705No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC14800.3804891342072586No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA14470.3720052548634481No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA12870.33087129440860935No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT11740.30182043483737947No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC11600.29822121329758106No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11470.2948790790106254No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10590.27225540076046406No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT10340.26582821943939555No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT10310.2650569576808673No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC9410.24191910492502047No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC9280.23857697063806488No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9110.23420648733973823No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8780.22572260799592772No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT8690.22340882272034304No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG8390.2156962051350608No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA8220.2113257218367342No Hit
AGTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG8020.20618397677987932No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7970.2048985405156656No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7840.20155640622870996No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATACAG7560.19435796314911316No Hit
ATATGAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7410.19050165435647204No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7370.18947330534510107No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7340.18870204358657283No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7300.1876736945752019No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7030.18073233874844782No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6530.16787797610631072No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6400.16453584181935507No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6320.16247914379661313No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6290.1617078820380849No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6250.16067953302671395No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6170.158622835003972No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6150.15810866049828654No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6140.15785157324544377No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6120.15733739873975827No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6080.15630904972838733No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5970.15348108994711715No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5960.1532240026942744No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT5920.15219565368290344No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5850.15039604291300424No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5830.14988186840731876No Hit
GGTTTGATACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT5750.14782517038457682No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5700.1465397341203631No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5510.141655076316351No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5460.14036964005213728No Hit
GTTTAAATGTATACCCAAAGACAGAAGAAAATTGGTAACAGCGGTAAAAA5350.1375416802708671No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5340.1372845930180244No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5320.1367704185123389No Hit
GTATTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAA5250.1349708077424397No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5200.13368537147822598No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5150.13239993521401228No Hit
TTTCCGGCTCCTCTGCCGATCCATACCGCGGAACTCCTAGCCGCTTGTTT5080.13060032444411307No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC5060.1300861499384276No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACG5030.12931488817989936No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGCTTT5020.12905780092705665No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC4970.12777236466284292No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4960.12751527741000018No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC4950.12725819015715745No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4950.12725819015715745No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4940.12700110290431468No Hit
AGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC4900.12597275389294374No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4900.12597275389294374No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4880.12545857938725824No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4880.12545857938725824No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCCGTCCCGAGAATGTTT4800.12340188136451631No Hit
GGGAAATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4740.12185935784745985No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4740.12185935784745985No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4630.11903139806618969No Hit
GAGTTATCCGTCCCGAGAATGTTTGCCCCAGACCTGCTGCGAGCAAAACA4620.11877431081334694No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4610.11851722356050419No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4530.11646052553776226No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4530.11646052553776226No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4480.11517508927354855No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC4450.11440382751502032No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4450.11440382751502032No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4430.11388965300933485No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4360.11209004223943564No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4350.1118329549865929No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4330.1113187804809074No Hit
GTATCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4280.1100333442166937No Hit
CCCAAAGACAGAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG4240.10900499520532274No Hit
GGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGA4210.10823373344679452No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4210.10823373344679452No Hit
GTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4200.10797664619395177No Hit
GGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTC4160.10694829718258081No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4140.10643412267689531No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4130.10617703542405256No Hit
GATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4120.10591994817120982No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTCTGTC4050.10412033740131064No Hit
GAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC3990.10257781388425419No Hit
ACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG3980.10232072663141144No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC3980.10232072663141144No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGGCAGACGGAGAAGG3970.10206363937856869No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGT3970.10206363937856869No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3950.1015494648728832No Hit
GTATATTTGCGGGAGAGGACAACAGAGTTATCCGTCCCGAGAATGTTTGC3940.10129237762004047No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3910.10052111586151223No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG3900.10026402860866948No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3890.10000694135582676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)