FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418893
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGA27300.6517177417622162No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC18040.4306589033476329No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13520.32275545306319275No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12280.29315362156923125No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACGATACGTTGACAGACT11330.27047479905369626No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11280.2692811768160365No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT9600.22917546963066943No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9530.2275043984979458No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT9340.2229686339948388No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8970.21413582943615672No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8950.21365838054109282No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8940.2134196560935609No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8930.21318093164602894No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGT8910.21270348275096504No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8690.20745154490526221No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8410.20076726037436768No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTT8300.19814129145151624No Hit
CTCCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA8040.19193445581568563No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT7980.19050210913049395No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7730.18453399794219527No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7630.18214675346687578No Hit
GTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCGGGAT7480.1785658867538966No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7440.17761098896376878No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7430.17737226451623683No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7140.17044925553781037No Hit
GGATAAGGGTATTAAACCTTATTATCCCGAACATCTAGTTAATCATTACT6940.16567476658717142No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6910.1649585932445756No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAATCTAATCTCCTCC6850.16352624655938391No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6760.16137772653159638No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6730.16066155318900055No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6710.16018410429393665No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCCAGAAGATCTCGTA6500.15517089089576575No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA6210.14824788191733926No Hit
GTATTAAACCTTATTATCCCGAACATCTAGTTAATCATTACTTCCAAACT6110.1458606374420198No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6020.14371211741423226No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5950.14204104628150863No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTTGGA5940.14180232183397667No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACGAT5810.1386989040160614No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5730.1367891084358058No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5660.13511803730308217No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5620.1341631395129544No Hit
GTTATGTTAACACTCATATGGGCCTAAAGTTCAGACAACTATTGTGGTTT5530.13201461948516685No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGT5430.1296273750098474No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5400.12891120166725153No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAATCTAATCT5230.12485288605920844No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5010.11960094821350559No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTCATATGGGC4920.11745242818571808No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4810.11482645926286666No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4800.11458773481533471No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4720.11267793923507913No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4590.10957452141716381No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG4580.10933579696963186No Hit
GTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCGGG4580.10933579696963186No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC4550.10861962362703603No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4550.10861962362703603No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4530.10814217473197213No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4500.10742600138937629No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4460.1064711035992485No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4430.10575493025665265No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC4390.10480003246652486No Hit
GTCATAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC4340.10360641022886513No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGCC4340.10360641022886513No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4240.10121916575354566No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC4210.1005029924109498No Hit
CTAGTAAACTGAGCCATGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4190.10002554351588593No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4190.10002554351588593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)