FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601491
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA48760.8106521959597068No Hit
CTCTAAGACCGTGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA31350.5212048060569485No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT24690.4104799573060943No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23260.3867057029947248No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16670.2771446289304412No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT16330.2714920090242414No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG15080.2507103181926247No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT14830.2465539800263013No Hit
GTGCTGGTGCGCAAACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14230.23657876842712527No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT14200.23608000784716646No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC14080.23408496552733124No Hit
TAATAACTCTATCCACCTGGGTGGGTGGTAATTTGGAAGATCCAACATCC14050.23358620494737242No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT13830.2299286273610079No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC13580.22577228919468456No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13250.22028592281513773No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT13040.21679459875542612No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12950.21529831701554972No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC12610.20964569710934994No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC12360.20548935894302658No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12060.20050175314343857No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11730.19501538676389174No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT11660.19385161207732118No Hit
GTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAATACGTTGACAGACT11590.19268783739075065No Hit
GTTTCCTAAAACATTCTTTGATTTTTTGTATGATGTGATCTTGTGGCAAT11560.19218907681079186No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10640.1768937523587219No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT10620.17656124530541603No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10590.17606248472545724No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGAATTGGTGGGCGTTC10470.17406744240562202No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT10450.17373493535231618No Hit
CCTAATAACTCTATCCACCTGGGTGGGTGGTAATTTGGAAGATCCAACAT10080.16758355486615759No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG10050.1670847942861988No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGAAT10010.16641978017958706No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC9980.16592101959962824No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9950.16542225901966945No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9890.16442473785975184No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9840.16359347022648718No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG9480.15760834326698156No Hit
ATGTAAGACCTTAGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9110.151456962780823No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT9110.151456962780823No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA9090.15112445572751712No Hit
CTTTTACACAATGTGGGTATCCTGCTTTAAAGCCTTTGTATGCATGTATT9020.14996068104094656No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA8780.14597059640127616No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC8630.14347679350148215No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT8600.14297803292152333No Hit
CTACAAACCTTGCCAACAAATCCACCTCCTGCCTCTACCAATCGCCAGTC8440.1403179764950764No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8370.13915420180850585No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC8340.13865544122854706No Hit
CCCTAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG8240.1369929059620177No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT8030.1335015819023061No Hit
GTATCCAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGA7950.13217155368908262No Hit
AATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT7730.12851397610271809No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG7560.12568766614961818No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7430.12352637030313006No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7330.12186383503660073No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7270.12086631387668312No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7240.1203675532967243No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT7210.11986879271676551No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAACCCAAGAGACCCACAATACG7200.11970253919011258No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC7130.11853876450354202No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7080.11770749687027736No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7060.1173749898169715No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA7060.1173749898169715No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7040.11704248276366562No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6920.1150474404438304No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAC6740.11205487696407761No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTAAAAGCCAAA6690.11122360933081293No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6630.11022608817089534No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6600.10972732759093652No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6560.10906231348432478No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA6530.10856355290436598No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC6510.10823104585106012No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6510.10823104585106012No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC6500.10806479232440717No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG6420.10673476411118372No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA6410.10656851058453078No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA6400.10640225705787784No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6390.1062360035312249No Hit
CTCTAAGGCTTCCCGAAACAGAGCTGAGGCGGTATCCAGAAGATCTCGTA6380.10606975000457197No Hit
TTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGT6370.10590349647791902No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCTAAAAGGCCTCCGT6350.10557098942461317No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG6330.1052384823713073No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6300.10473972179134851No Hit
ATCCAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6240.1037422006314309No Hit
GTACAAAGATCATTAATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTA6230.10357594710477797No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTCTGTGGGTCACCATATT6230.10357594710477797No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6190.10291093299816623No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6080.10108214420498395No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAAAAA6070.10091589067833101No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC6020.10008462304506635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)